Adropin Polyclonal antibody

Adropin Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 25213-1-AP

Synonyms

Adropin, C9orf165, energy homeostasis associated, ENHO, UNQ470

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Validation Data Gallery

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  IHC staining of human liver using 25213-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25213-1-AP (Adropin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 25213-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25213-1-AP (Adropin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of SH-SY5Y using 25213-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Adropin antibody (25213-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: SH-SY5Y cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25213-1-AP targets Adropin in IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Adropin fusion protein Ag19526
• Full Name: energy homeostasis associated
• Calculated Molecular Weight: 75 aa, 8 kDa
• GenBank Accession Number: BC022101
• Gene Symbol: ENHO
• Gene ID (NCBI): 375704
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Adropin is a nutritionally regulated peptide hormone that is primarily secreted by the liver and is important in maintaining metabolic and non-metabolic homeostasis. Adropin regulates glucose and fatty acid metabolism and exerts a potential protective effect on endothelial function through modulation of endothelial nitric oxide (NO) synthesis. It is associated with obesity and is involved in the prevention of insulin resistance, dyslipidemia, and impaired glucose tolerance (PMID: 22318315).