ATL3 Monoclonal antibody

ATL3 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2E7C4

Cat no : 68516-1-Ig

Synonyms

ATL3, Atlastin 3, atlastin GTPase 3, DKFZp564J0863

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Validation Data Gallery

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  WB analysis using 68516-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68516-1-Ig (ATL3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: PC-3 cells, HeLa cells, Jurkat cells, K-562 cells, rabbit testis tissue, rat testis tissue, mouse testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68516-1-Ig targets ATL3 in WB, ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.

• Tested Reactivity: Human, mouse, rat, rabbit
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ATL3 fusion protein Ag10248
• Full Name: atlastin GTPase 3
• Calculated Molecular Weight: 541 aa, 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC077727
• Gene Symbol: ATL3
• Gene ID (NCBI): 25923
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATL3, belonging to a class of dynamin-like GTPase, was reported to mediate ER fusion. ATL3 is required for the proper formation of the network of interconnected tubules of the endoplasmic reticulum. ATL3 functions as a receptor for ER-phagy, promoting tubular ER degradation upon starvation. Mutations in this gene may be associated with hereditary sensory neuropathy type IF.