ATL3 Polyclonal antibody

• Featured Product
• KD/KO Validated

ATL3 Polyclonal Antibody for WB, IP, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

11

Cat no : 16921-1-AP

Synonyms

ATL3, Atlastin 3, atlastin GTPase 3, Atlastin-3

---

Validation Data Gallery

• 

  WB analysis using 16921-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16921-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 antibody) at dilution of 1:9000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SMMC-7721 using 16921-1-AP

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16921-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16921-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16921-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16921-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16921-1-AP (ATL3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16921-1-AP

  IP result of anti-ATL3 (IP:16921-1-AP, 4ug; Detection:16921-1-AP 1:5000) with HeLa cells lysate 1720 ug.

• 

  IP experiment of HeLa using 16921-1-AP

  IP result of anti-ATL3 (IP:16921-1-AP, 4ug; Detection:16921-1-AP 1:8000) with HeLa cells lysate 1760 ug.

• 

  IF Staining of HeLa using 16921-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ATL3 antibody (16921-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 16921-1-AP

  Immunofluorescent analysis of HepG2 cells, using ATL3 antibody 16921-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, mouse liver tissue, HEK-293 cells, HepG2 cells, SMMC-7721 cells, Jurkat cells, NIH/3T3 cells, rat liver tissue
• Positive IP detected in: HeLa cells
• Positive IF detected in: HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 10 publications below
• IF: See 2 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

16921-1-AP targets ATL3 in WB, IP, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, monkey
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATL3 fusion protein Ag10279
• Full Name: atlastin GTPase 3
• Calculated Molecular Weight: 541 aa, 61 kDa
• Observed Molecular Weight: 61 kDa
• GenBank Accession Number: BC077727
• Gene Symbol: ATL3
• Gene ID (NCBI): 25923
• RRID: AB_2290228
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATL3, belonging to a class of dynamin-like GTPase, was reported to mediate ER fusion. ATL3 is required for the proper formation of the network of interconnected tubules of the endoplasmic reticulum. ATL3 functions as a receptor for ER-phagy, promoting tubular ER degradation upon starvation. Mutations in this gene may be associated with hereditary sensory neuropathy type IF.

Publications

Species	Application	Title
human	WB,IP	Nature; ER proteins decipher the tubulin code to regulate organelle distribution; Authors - Pengli Zheng
human,mouse	WB	Cell Res; DNA damage triggers tubular endoplasmic reticulum extension to promote apoptosis by facilitating ER-mitochondria signaling.; Authors - Pengli Zheng
	WB	Genes Dev; Localization of Kif1c mRNA to cell protrusions dictates binding partner specificity of the encoded protein; Authors - Megan L Norris
human	WB,IF	Elife; Efficient protein targeting to the inner nuclear membrane requires Atlastin-dependent maintenance of ER topology.; Authors - Sumit Pawar ; KD Validated
mouse	WB	Nature; Heteromeric clusters of ubiquitinated ER-shaping proteins drive ER-phagy; Authors - Hector Foronda
mouse	WB	Neuron; Profiling of purified autophagic vesicle degradome in the maturing and aging brain; Authors - Emmanouela Kallergi