CoraLite® Plus 488-conjugated ASH2L Monoclonal antibody

ASH2L Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1G1H3

Cat no : CL488-67859

Synonyms

ASH2, ASH2 like protein, ASH2L, ASH2L1, ASH2L2, Bre2

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Validation Data Gallery

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  FC experiment of HeLa using CL488-67859

  1X10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human ASH2L (CL488-67859, Clone:1G1H3) (red), or 0.8 ug CoraLite® Plus 488 Mouse IgG2a Isotype Control (C1.18.4) (CL488-65208, Clone: C1.18.4) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive FC detected in: HeLa cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67859 targets ASH2L in FC (Intra) applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: ASH2L fusion protein Ag3008
• Full Name: ash2 (absent, small, or homeotic)-like (Drosophila)
• Calculated Molecular Weight: 628 aa, 69 kDa
• Observed Molecular Weight: 80 kDa
• GenBank Accession Number: BC015936
• Gene Symbol: ASH2L
• Gene ID (NCBI): 9070
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Related Products

Foxp3 / Transcription Factor Staining Buffer Kit

PF00011