AQP2 Polyclonal antibody

AQP2 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 29386-1-AP

Synonyms

ADH water channel, AQP CD, AQP2, AQP-2, Aquaporin 2, aquaporin 2 (collecting duct), Aquaporin CD, WCH CD

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Validation Data Gallery

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  WB analysis of mouse kidney using 29386-1-AP

  37°C incubated mouse kidney tissue were subjected to SDS PAGE followed by western blot with 29386-1-AP (AQP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 29386-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29386-1-AP (AQP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 29386-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 29386-1-AP (AQP2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse kidney using 29386-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse kidney tissue using AQP2 antibody (29386-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), AQP1 antibody (66805-1-Ig, Clone: 2E5D6, green).

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Tested Applications

• Positive WB detected in: 37°C incubated mouse kidney tissue
• Positive IHC detected in: mouse kidney tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

29386-1-AP targets AQP2 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.

• Tested Reactivity: Human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: AQP2 fusion protein Ag29931
• Full Name: aquaporin 2 (collecting duct)
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 28-29, 35-38 kDa
• GenBank Accession Number: NM_000486
• Gene Symbol: AQP2
• Gene ID (NCBI): 359
• RRID: AB_2918288
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

AQP2 (Aquaporin2) is a water channel protein that is widely distributed among mammalian tissues and plays a major role in water homeostasis. AQP2 is mainly found in the apical cell membranes of the kidney's collecting duct cells and is critical for urinary concentration. AQP2 can be glycosylated and this antibody recognizes the 28-29 kDa non-glycosylated as well as the 35-40 kDa glycosylated forms of AQP2.