ALDH1L1 Monoclonal antibody

ALDH1L1 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse, pig

Applications

WB, IHC

Conjugate

Unconjugated

CloneNo.

1C1A3

Cat no : 68018-1-Ig

Synonyms

10 FTHFDH, ALDH1L1, DKFZp781N0997, FTHFD

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Validation Data Gallery

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  WB analysis of human colostrum using 68018-1-Ig

  human colostrum were subjected to SDS PAGE followed by western blot with 68018-1-Ig (ALDH1L1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig liver using 68018-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 68018-1-Ig (ALDH1L1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 68018-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 68018-1-Ig (ALDH1L1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 68018-1-Ig

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 68018-1-Ig (ALDH1L1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 68018-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68018-1-Ig (ALDH1L1 antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 68018-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68018-1-Ig (ALDH1L1 antibody) at dilution of 1:32000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: human colostrum, pig liver tissue, rat liver tissue, mouse liver tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:16000-1:64000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68018-1-Ig targets ALDH1L1 in WB, IHC applications and shows reactivity with Human, rat, mouse, pig samples.

• Tested Reactivity: Human, rat, mouse, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: ALDH1L1 fusion protein Ag13190
• Full Name: aldehyde dehydrogenase 1 family, member L1
• Calculated Molecular Weight: 99 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC027241
• Gene Symbol: ALDH1L1
• Gene ID (NCBI): 10840
• RRID: AB_2918764
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Aldehyde dehydrogenase 1 family member L1 (ALDH1L1), belongs to the aldehyde dehydrogenase family of proteins and is responsible for formate oxidation in vivo. Loss of ALDH1L1 is associated with decreased apoptosis, increased cell motility, and cancer progression. ALDH1L1 catalyzes the conversion of 10-formyltetrahydrofolate, nicotinamide adenine dinucleotide phosphate (NADP+), and water to tetrahydrofolate, NADPH, and carbon dioxide. ALDH1L1 has some isoforms with MW of 99, 88 and 55 kDa.