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Maspin Polyclonal antibody

Maspin Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11722-1-AP

Synonyms

SERPINB5, SERPINB 5, Serpin B5, PI-5, PI5

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using Maspin Polyclonal antibody (11722-1-AP)

    WB analysis of HepG2 using 11722-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11722-1-AP (Maspin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using Maspin Polyclonal antibody (11722-1-AP)

    WB analysis of A431 using 11722-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 11722-1-AP (Maspin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using Maspin Polyclonal antibody (11722-1-AP)

    WB analysis of HeLa using 11722-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11722-1-AP (Maspin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Maspin Polyclonal antibody (11722-1-AP)

    IHC staining of human lung cancer using 11722-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11722-1-AP (Maspin antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human bowen disease using Maspin Polyclonal antibody (11722-1-AP)

    IHC staining of human bowen disease using 11722-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11722-1-AP (Maspin antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human bowen disease using Maspin Polyclonal antibody (11722-1-AP)

    IHC staining of human bowen disease using 11722-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 11722-1-AP (Maspin antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using Maspin Polyclonal antibody (11722-1-AP)

    IHC staining of human pancreas using 11722-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11722-1-AP (Maspin antibody at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using Maspin Polyclonal antibody (11722-1-AP)

    IHC staining of human pancreas using 11722-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 11722-1-AP (Maspin antibody at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Maspin Polyclonal antibody (11722-1-AP)

    IHC staining of human breast cancer using 11722-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 11722-1-AP (Maspin antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Maspin Polyclonal antibody (11722-1-AP)

    IF Staining of A431 using 11722-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using Maspin antibody (11722-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, A431 cells, HeLa cells
Positive IHC detected inhuman bowen disease, human breast cancer tissue, human lung cancer tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11722-1-AP targets Maspin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Maspin fusion protein Ag2304
Full Name serpin peptidase inhibitor, clade B (ovalbumin), member 5
Calculated Molecular Weight 375 aa, 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC020713
Gene Symbol Maspin
Gene ID (NCBI) 5268
RRIDAB_10597111
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SERPINB5, also named as PI-5, Maspin and Serpin B5, belongs to the serpin family and Ov-serpin subfamily. It is a tumor suppressor. It blocks the growth, invasion, and metastatic properties of mammary tumors. As it does not undergo the S (stressed) to R (relaxed) conformational transition characteristic of active serpins, SERPINB5 exhibits no serine protease inhibitory activity.

Protocols

Product Specific Protocols
WB protocol for Maspin antibody 11722-1-APDownload protocol
IHC protocol for Maspin antibody 11722-1-APDownload protocol
IF protocol for Maspin antibody 11722-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Int J Cancer

Serpin B5 is a CEA-interacting biomarker for colorectal cancer.

Authors - Baek Ji Yeon JY
View Article
humanWB

Cancer Lett

Cancer stem-like cell characteristics induced by EB virus-encoded LMP1 contribute to radioresistance in nasopharyngeal carcinoma by suppressing the p53-mediated apoptosis pathway.

Authors - Chang-Fu Yang
View Article
humanWB

Oncol Lett

iTRAQ-coupled 2D LC/MS-MS analysis of CXCR7-transfected papillary thyroid carcinoma cells: A new insight into CXCR7 regulation of papillary thyroid carcinoma progression and identification of potential biomarkers.

Authors - Hengwei Zhang
View Article
humanIF

Cell Death Dis

FAT10 promotes chemotherapeutic resistance in pancreatic cancer by inducing epithelial-mesenchymal transition via stabilization of FOXM1 expression.

Authors - Jinfeng Zhu
View Article
humanIHC

J Thorac Dis

Exploring diverse programmed cell-death patterns to develop a novel gene signature for predicting the prognosis of lung adenocarcinoma patients

Authors - Zhanming Ma
View Article