SCEL Polyclonal antibody

SCEL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11906-1-AP

Synonyms

Sciellin



Tested Applications

Positive WB detected inhuman saliva tissue, A431 cells, human skin tissue, mouse skin tissue
Positive IHC detected inhuman renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11906-1-AP targets SCEL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SCEL fusion protein Ag2578
Full Name sciellin
Calculated Molecular Weight 668 aa, 75 kDa
Observed Molecular Weight 70-78 kDa
GenBank Accession NumberBC020726
Gene Symbol SCEL
Gene ID (NCBI) 8796
RRIDAB_2183262
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SCEL antibody 11906-1-APDownload protocol
IHC protocol for SCEL antibody 11906-1-APDownload protocol
IF protocol for SCEL antibody 11906-1-APDownload protocol
FC protocol for SCEL antibody 11906-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols