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SCEL Polyclonal antibody

SCEL Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 11906-1-AP

Synonyms

Sciellin

Validation Data Gallery

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  • Western Blot (WB) analysis of human saliva using SCEL Polyclonal antibody (11906-1-AP)

    WB analysis of human saliva using 11906-1-AP

    human saliva were subjected to SDS PAGE followed by western blot with 11906-1-AP (SCEL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human skin tissue using SCEL Polyclonal antibody (11906-1-AP)

    WB analysis of human skin using 11906-1-AP

    human skin tissue were subjected to SDS PAGE followed by western blot with 11906-1-AP (SCEL antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using SCEL Polyclonal antibody (11906-1-AP)

    WB analysis of A431 using 11906-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 11906-1-AP (SCEL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using SCEL Polyclonal antibody (11906-1-AP)

    WB analysis using 11906-1-AP

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 11906-1-AP (SCEL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using SCEL Polyclonal antibody (11906-1-AP)

    IHC staining of human renal cell carcinoma using 11906-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 11906-1-AP (SCEL antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human renal cell carcinoma tissue using SCEL Polyclonal antibody (11906-1-AP)

    IHC staining of human renal cell carcinoma using 11906-1-AP

    Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 11906-1-AP (SCEL antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SCEL Polyclonal antibody (11906-1-AP)

    IF Staining of HepG2 using 11906-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 11906-1-AP (SCEL antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using SCEL Polyclonal antibody (11906-1-AP)

    FC experiment of HeLa using 11906-1-AP

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug SCEL Polyclonal antibody (11906-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inhuman saliva tissue, A431 cells, human skin tissue, mouse skin tissue
Positive IHC detected inhuman renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11906-1-AP targets SCEL in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SCEL fusion protein Ag2578
Full Name sciellin
Calculated Molecular Weight 668 aa, 75 kDa
Observed Molecular Weight 70-78 kDa
GenBank Accession NumberBC020726
Gene Symbol SCEL
Gene ID (NCBI) 8796
RRIDAB_2183262
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SCEL antibody 11906-1-APDownload protocol
IHC protocol for SCEL antibody 11906-1-APDownload protocol
IF protocol for SCEL antibody 11906-1-APDownload protocol
FC protocol for SCEL antibody 11906-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols