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  • Featured Product
  • KD/KO Validated

RPS21 Polyclonal antibody

RPS21 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16946-1-AP

Synonyms

Small ribosomal subunit protein eS21, ribosomal protein S21, 40S ribosomal protein S21

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPS21 Polyclonal antibody (16946-1-AP)

    WB analysis using 16946-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16946-1-AP (RPS21 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RPS21 Polyclonal antibody (16946-1-AP)

    WB analysis using 16946-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16946-1-AP (RPS21 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using RPS21 Polyclonal antibody (16946-1-AP)

    WB analysis of HepG2 using 16946-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 16946-1-AP (RPS21 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of SGC-7901 cells using RPS21 Polyclonal antibody (16946-1-AP)

    WB analysis of SGC-7901 using 16946-1-AP

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 16946-1-AP (RPS21 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RPS21 Polyclonal antibody (16946-1-AP)

    IHC staining of human liver cancer using 16946-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16946-1-AP (RPS21 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RPS21 Polyclonal antibody (16946-1-AP)

    IHC staining of human liver cancer using 16946-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16946-1-AP (RPS21 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RPS21 Polyclonal antibody (16946-1-AP)

    IF Staining of HepG2 using 16946-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPS21 antibody (16946-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, mouse liver tissue, SGC-7901 cells, rat liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16946-1-AP targets RPS21 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPS21 fusion protein Ag10557
Full Name ribosomal protein S21
Calculated Molecular Weight 81 aa, 9 kDa
Observed Molecular Weight 9 kDa
GenBank Accession NumberBC018140
Gene Symbol RPS21
Gene ID (NCBI) 6227
RRIDAB_2180351
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit.

Protocols

Product Specific Protocols
WB protocol for RPS21 antibody 16946-1-APDownload protocol
IHC protocol for RPS21 antibody 16946-1-APDownload protocol
IF protocol for RPS21 antibody 16946-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Oncol Lett

Identification of the key genes and pathways in prostate cancer.

Authors - Shutong Fan
View Article
humanWB,IHC

Med Oncol

Identification of candidate diagnostic and prognostic biomarkers for human prostate cancer: RPL22L1 and RPS21.

Authors - Zumu Liang
  • KD Validated
View Article
mouseIHC

Reprod Sci

Upregulated Ribosomal Pathway Impairs Follicle Development in a Polycystic Ovary Syndrome Mouse Model: Differential Gene Expression Analysis of Oocytes

Authors - Natsuki Nakanishi
View Article