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  • Featured Product
  • KD/KO Validated

RPL39 Polyclonal antibody

RPL39 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14990-1-AP

Synonyms

ribosomal protein L39, Large ribosomal subunit protein eL39, eL39, 60S ribosomal protein L39

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using RPL39 Polyclonal antibody (14990-1-AP)

    WB analysis of HepG2 using 14990-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14990-1-AP (RPL39 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human liver tissue using RPL39 Polyclonal antibody (14990-1-AP)

    WB analysis of human liver using 14990-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 14990-1-AP (RPL39 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RPL39 Polyclonal antibody (14990-1-AP)

    IHC staining of human liver cancer using 14990-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 14990-1-AP (RPL39 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using RPL39 Polyclonal antibody (14990-1-AP)

    IHC staining of human liver cancer using 14990-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 14990-1-AP (RPL39 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RPL39 Polyclonal antibody (14990-1-AP)

    IF Staining of HepG2 using 14990-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RPL39 antibody (14990-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHepG2 cells, human liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14990-1-AP targets RPL39 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPL39 fusion protein Ag6975
Full Name ribosomal protein L39
Calculated Molecular Weight 6 kDa
Observed Molecular Weight 6 kDa
GenBank Accession NumberBC001019
Gene Symbol RPL39
Gene ID (NCBI) 6170
RRIDAB_2238670
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RPL39 antibody 14990-1-APDownload protocol
IHC protocol for RPL39 antibody 14990-1-APDownload protocol
IF protocol for RPL39 antibody 14990-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

Early Scanning of Nascent Polypeptides inside the Ribosomal Tunnel by NAC.

Authors - Martin Gamerdinger
View Article
humanFC

Transl Res

Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication.

Authors - Mahmoud Dabbah
View Article
zebrafishIF

Elife

In vivo proximity labeling identifies cardiomyocyte protein networks during zebrafish heart regeneration.

Authors - Mira I Pronobis
View Article
humanIF

Cell Discov

Ribosomal RNA regulates chromosome clustering during mitosis.

Authors - Kai Ma
View Article
humanWB,IHC,IF

Front Immunol

Identification and validation of a 17-gene signature to improve the survival prediction of gliomas

Authors - Shiao Tong
  • KD Validated
View Article
mouseWB,IP,IF

iScience

Proteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis.

Authors - Qianxing Zou
  • KO Validated
View Article