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RPL37A Polyclonal antibody

RPL37A Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 14660-1-AP

Synonyms

ribosomal protein L37a, Large ribosomal subunit protein eL43, 60S ribosomal protein L37a

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPL37A Polyclonal antibody (14660-1-AP)

    WB analysis using 14660-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14660-1-AP (RPL37A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using RPL37A Polyclonal antibody (14660-1-AP)

    IP experiment of MCF-7 using 14660-1-AP

    IP result of anti-RPL37A (IP:14660-1-AP, 4ug; Detection:14660-1-AP 1:500) with MCF-7 cells lysate 3480 ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RPL37A Polyclonal antibody (14660-1-AP)

    IHC staining of human breast cancer using 14660-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14660-1-AP (RPL37A antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RPL37A Polyclonal antibody (14660-1-AP)

    IHC staining of human breast cancer using 14660-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 14660-1-AP (RPL37A antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of SH-SY5Y cells using RPL37A Polyclonal antibody (14660-1-AP)

    IF Staining of SH-SY5Y using 14660-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 14660-1-AP (RPL37A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Flow cytometry (FC) experiment of MCF-7 cells using RPL37A Polyclonal antibody (14660-1-AP)

    FC experiment of MCF-7 using 14660-1-AP

    1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human RPL37A (14660-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inCOLO 320 cells, HeLa cells, MCF-7 cells, SH-SY5Y cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14660-1-AP targets RPL37A in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPL37A fusion protein Ag6330
Full Name ribosomal protein L37a
Calculated Molecular Weight 10 kDa
Observed Molecular Weight 10 kDa
GenBank Accession NumberBC063476
Gene Symbol RPL37A
Gene ID (NCBI) 6168
RRIDAB_2238668
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RPL37A antibody 14660-1-APDownload protocol
IHC protocol for RPL37A antibody 14660-1-APDownload protocol
IF protocol for RPL37A antibody 14660-1-APDownload protocol
IP protocol for RPL37A antibody 14660-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanFC

Transl Res

Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication.

Authors - Mahmoud Dabbah
View Article
humanWB

iScience

TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment

Authors - Magdalena Wolczyk
View Article
humanWB

Cell

A non-canonical role for a small nucleolar RNA in ribosome biogenesis and senescence

Authors - Yujing Cheng
View Article