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RPL13A Polyclonal antibody

RPL13A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14633-1-AP

Synonyms

ribosomal protein L13a, Large ribosomal subunit protein uL13

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RPL13A Polyclonal antibody (14633-1-AP)

    WB analysis using 14633-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14633-1-AP (RPL13A antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using RPL13A Polyclonal antibody (14633-1-AP)

    WB analysis of Jurkat using 14633-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14633-1-AP (RPL13A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using RPL13A Polyclonal antibody (14633-1-AP)

    WB analysis of A549 using 14633-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 14633-1-AP (RPL13A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using RPL13A Polyclonal antibody (14633-1-AP)

    IHC staining of human stomach cancer using 14633-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 14633-1-AP (RPL13A antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inA549 cells, Jurkat cells, HEK-293 cells, HeLa cells, RAW264.7 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14633-1-AP targets RPL13A in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPL13A fusion protein Ag6212
Full Name ribosomal protein L13a
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 25-28 kDa
GenBank Accession NumberBC065236
Gene Symbol RPL13A
Gene ID (NCBI) 23521
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ribosomal protein L13a(RPL13a) is a member of the L13 ribosomal protein family and a structural component of the large 60S ribosomal subunit. It's a reference gene(RG) that stably expressed not only during differentiation, but also during expansion to higher passages. And it's suggested as a RG for a variety research field. RPL13a is a key component of the IFN-gamma-activated inhibitor of translation(GAIT) complex, which inhibits translation of a select group of inflammation-related transcripts via binding to rhe 3' UTR of target mRNA.once phosphorylated of Ser77 by ZIPK, RPL13a released from the complex.

Protocols

Product Specific Protocols
WB protocol for RPL13A antibody 14633-1-APDownload protocol
IHC protocol for RPL13A antibody 14633-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols