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RPL12 Polyclonal antibody

RPL12 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 14536-1-AP

Synonyms

60S ribosomal protein L12, ribosomal protein L12, RPL12

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using RPL12 Polyclonal antibody (14536-1-AP)

    WB analysis using 14536-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14536-1-AP (RPL12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using RPL12 Polyclonal antibody (14536-1-AP)

    WB analysis of Jurkat using 14536-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14536-1-AP (RPL12 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using RPL12 Polyclonal antibody (14536-1-AP)

    WB analysis of A549 using 14536-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 14536-1-AP (RPL12 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inA549 cells, Jurkat cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

14536-1-AP targets RPL12 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPL12 fusion protein Ag6037
Full Name ribosomal protein L12
Calculated Molecular Weight 18 kDa
Observed Molecular Weight 21 kDa
GenBank Accession NumberBC050644
Gene Symbol RPL12
Gene ID (NCBI) 6136
RRIDAB_2269722
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RPL12 antibody 14536-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Cell Biol

CDK1 couples proliferation with protein synthesis.

Authors - Katharina Haneke
View Article
humanWB

Sci Rep

RNA G-quadruplex structures control ribosomal protein production

Authors - DhavalVarshney
View Article