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RBM22 Polyclonal antibody

RBM22 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 22103-1-AP

Synonyms

RNA-binding motif protein 22, RNA binding motif protein 22, Pre-mRNA-splicing factor RBM22, Pre mRNA splicing factor RBM22, fSAP47

Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using RBM22 Polyclonal antibody (22103-1-AP)

    WB analysis of Raji using 22103-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 22103-1-AP (RBM22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using RBM22 Polyclonal antibody (22103-1-AP)

    WB analysis using 22103-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22103-1-AP (RBM22 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using RBM22 Polyclonal antibody (22103-1-AP)

    WB analysis of NIH/3T3 using 22103-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22103-1-AP (RBM22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using RBM22 Polyclonal antibody (22103-1-AP)

    IF Staining of U2OS using 22103-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using RBM22 antibody (22103-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Tested Applications

Positive WB detected inNIH/3T3 cells, Raji cells
Positive IF/ICC detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22103-1-AP targets RBM22 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RBM22 fusion protein Ag17315
Full Name RNA binding motif protein 22
Calculated Molecular Weight 420 aa, 47 kDa
Observed Molecular Weight 47-50 kDa
GenBank Accession NumberBC003402
Gene Symbol RBM22
Gene ID (NCBI) 55696
RRIDAB_2878996
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RBM22 is mainly involved in pre-mRNA splicing, playing the essential role of maintaining the conformation of the catalytic core of the spliceosome and acting as a bridge between the catalytic core and other essential protein components of the spliceosome. RBM22 is also involved in gene regulation, and is able to bind DNA, acting as a bona fide transcription factor on a large number of target genes. Due to its wide scope in the regulation of gene expression, RBM22 has been associated with several pathologies and, notably, with the aggressiveness of cancer cells and with the phenotype of a myelodysplastic syndrome. (PMID: 35158909)

Protocols

Product Specific Protocols
WB protocol for RBM22 antibody 22103-1-APDownload protocol
IF protocol for RBM22 antibody 22103-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Zhongwen (Verified Customer) (09-25-2023)

Good quality

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000