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  • Featured Product
  • KD/KO Validated

RAD17 Polyclonal antibody

RAD17 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13358-1-AP

Synonyms

RF-C/activator 1 homolog, R24L, HRAD17

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using RAD17 Polyclonal antibody (13358-1-AP)

    WB analysis using 13358-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13358-1-AP (RAD17 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RAD17 Polyclonal antibody (13358-1-AP)

    IHC staining of human breast cancer using 13358-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 13358-1-AP (RAD17 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using RAD17 Polyclonal antibody (13358-1-AP)

    IHC staining of human breast cancer using 13358-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 13358-1-AP (RAD17 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RAD17 Polyclonal antibody (13358-1-AP)

    IF Staining of HeLa using 13358-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13358-1-AP (RAD17 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using RAD17 Polyclonal antibody (13358-1-AP)

    IF Staining of HeLa using 13358-1-AP

    Immunofluorescent analysis of Hela cells, using RAD17 antibody 13358-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG (green). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inK-562 cells, Raji cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13358-1-AP targets RAD17 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RAD17 fusion protein Ag4171
Full Name RAD17 homolog (S. pombe)
Calculated Molecular Weight 681 aa, 71 kDa
Observed Molecular Weight 71 kDa
GenBank Accession NumberBC032304
Gene Symbol RAD17
Gene ID (NCBI) 5884
RRIDAB_2269268
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RAD17 antibody 13358-1-APDownload protocol
IHC protocol for RAD17 antibody 13358-1-APDownload protocol
IF protocol for RAD17 antibody 13358-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncogenesis

The TIP60-ATM axis regulates replication fork stability in BRCA-deficient cells.

Authors - Emily M Schleicher
  • KD Validated
View Article
mouseWB

Biochem J

Mutation of the RelA(p65) Thr505 phosphosite disrupts the DNA replication stress response leading to CHK1 inhibitor resistance

Authors - Jill E Hunter
View Article
mouseWB

Biochem J

Regulation of CHK1 inhibitor resistance by a c-Rel and USP1 dependent pathway

Authors - Jill E Hunter
View Article