- Featured Product
- KD/KO Validated
Liprin Alpha 1 Polyclonal antibody
Liprin Alpha 1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF/ICC, IP, ELISA
Conjugate
Unconjugated
Cat no : 14175-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells |
| Positive IP detected in | MCF-7 cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 10 publications below |
| WB | See 18 publications below |
| IHC | See 3 publications below |
| IF | See 20 publications below |
Product Information
14175-1-AP targets Liprin Alpha 1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, monkey |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Liprin Alpha 1 fusion protein Ag5381 |
| Full Name | protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 1 |
| Calculated Molecular Weight | 1202 aa, 136 kDa |
| Observed Molecular Weight | 140-160 kDa |
| GenBank Accession Number | BC034046 |
| Gene Symbol | Liprin Alpha 1 |
| Gene ID (NCBI) | 8500 |
| RRID | AB_2171592 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Liprin alpha 1, encoded by the PPFIA1 gene, is a member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family. Liprins interact with members of LAR family of transmembrane protein tyrosine phosphatases, which are known to be important for axon guidance and mammary gland development. Liprin alpha 1 may regulate the disassembly of focal adhesions and thus help orchestrate cell-matrix interactions (PMID: 7796809). Alternatively spliced transcript variants encoding distinct isoforms have been described.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Liprin Alpha 1 antibody 14175-1-AP | Download protocol |
| IHC protocol for Liprin Alpha 1 antibody 14175-1-AP | Download protocol |
| IF protocol for Liprin Alpha 1 antibody 14175-1-AP | Download protocol |
| IP protocol for Liprin Alpha 1 antibody 14175-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun Structural basis of liprin-α-promoted LAR-RPTP clustering for modulation of phosphatase activity. | ||
EMBO J The CEP170B-KIF2A complex destabilizes microtubule minus ends to generate polarized microtubule network
| ||
Nat Commun PPFIA1 drives active α5β1 integrin recycling and controls fibronectin fibrillogenesis and vascular morphogenesis.
| ||
Elife Local activation of focal adhesion kinase orchestrates the positioning of presynaptic scaffold proteins and Ca2+ signalling to control glucose-dependent insulin secretion. | ||
Mol Oncol Liprin-α1 contributes to oncogenic MAPK signaling by counteracting ERK activity
| ||
Stem Cells Transl Med Enhanced structure and function of human pluripotent stem cell-derived beta-cells cultured on extracellular matrix. |
