GIT1 Polyclonal antibody

GIT1 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 26247-1-AP

Synonyms

ARF GAP GIT1, CAT 1, CAT1, GIT1, GRK interacting protein 1



Tested Applications

Positive WB detected inROS1728 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 26247-1-AP is GIT1 Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GIT1 fusion protein Ag24648
Full Name G protein-coupled receptor kinase interacting ArfGAP 1
Calculated Molecular Weight 694 aa, 77 kDa
GenBank Accession NumberBC067358
Gene Symbol GIT1
Gene ID (NCBI) 28964
RRIDAB_2880445
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for GIT1 antibody 26247-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sammy (Verified Customer) (11-20-2023)

Detects and band above the expected kDa but has been validated by knock down. Antibody also works by immunofluorescence where it detects GIT1 at focal adhesions.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1 in 1000
  • Cell Tissue Type: HUVEC
GIT1 Antibody Western Blot validation (1 in 1000 dilution) in HUVEC (Cat no:26247-1-AP)