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PDCL2 Polyclonal antibody

PDCL2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17407-1-AP

Synonyms

GCPHLP, PDCL2, phosducin like 2, Phosducin like protein 2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PDCL2 Polyclonal antibody (17407-1-AP)

    WB analysis using 17407-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17407-1-AP (PDCL2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using PDCL2 Polyclonal antibody (17407-1-AP)

    WB analysis of mouse testis using 17407-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 17407-1-AP (PDCL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PDCL2 Polyclonal antibody (17407-1-AP)

    IHC staining of human kidney using 17407-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17407-1-AP (PDCL2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PDCL2 Polyclonal antibody (17407-1-AP)

    IHC staining of human kidney using 17407-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17407-1-AP (PDCL2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using PDCL2 Polyclonal antibody (17407-1-AP)

    IHC staining of human ovary using 17407-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17407-1-AP (PDCL2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using PDCL2 Polyclonal antibody (17407-1-AP)

    IHC staining of human ovary using 17407-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 17407-1-AP (PDCL2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PDCL2 Polyclonal antibody (17407-1-AP)

    IHC staining of human testis using 17407-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17407-1-AP (PDCL2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PDCL2 Polyclonal antibody (17407-1-AP)

    IHC staining of human testis using 17407-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 17407-1-AP (PDCL2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using PDCL2 Polyclonal antibody (17407-1-AP)

    IHC staining of human spleen using 17407-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 17407-1-AP (PDCL2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using PDCL2 Polyclonal antibody (17407-1-AP)

    IHC staining of human spleen using 17407-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 17407-1-AP (PDCL2 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inmouse testis tissue, rat testis tissue
Positive IHC detected inhuman kidney tissue, human ovary tissue, human spleen tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17407-1-AP targets PDCL2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDCL2 fusion protein Ag11427
Full Name phosducin-like 2
Calculated Molecular Weight 250 aa, 29 kDa
Observed Molecular Weight 30-35 kDa
GenBank Accession NumberBC034431
Gene Symbol PDCL2
Gene ID (NCBI) 132954
RRIDAB_2299492
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PDCL2 antibody 17407-1-APDownload protocol
IHC protocol for PDCL2 antibody 17407-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Andrology

PDCL2 is essential for sperm acrosome formation and male fertility in mice

Authors - Yoshitaka Fujihara
View Article
humanIHC

Transl Androl Urol

Identification of PDCL2 as a candidate marker in Sertoli cell-only syndrome by chromatin immunoprecipitation-sequencing and bioinformatics analysis

Authors - Yu Yang
View Article
mouseWB

PNAS Nexus

Tex46 knockout male mice are sterile secondary to sperm head malformations and failure to penetrate through the zona pellucida

Authors - Yoshitaka Fujihara
View Article