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MEA1 Polyclonal antibody

MEA1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10267-1-AP

Synonyms

HYS, male enhanced antigen 1, MEA, MEA 1, MEA1

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using MEA1 Polyclonal antibody (10267-1-AP)

    WB analysis of Jurkat using 10267-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10267-1-AP (MEA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using MEA1 Polyclonal antibody (10267-1-AP)

    WB analysis of mouse testis using 10267-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 10267-1-AP (MEA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using MEA1 Polyclonal antibody (10267-1-AP)

    IHC staining of human pancreas cancer using 10267-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10267-1-AP (MEA1 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inJurkat cells, mouse testis tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10267-1-AP targets MEA1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MEA1 fusion protein Ag0375
Full Name male-enhanced antigen 1
Calculated Molecular Weight 20 kDa
Observed Molecular Weight 34 kDa and 36 kDa
GenBank Accession NumberBC001754
Gene Symbol MEA1
Gene ID (NCBI) 4201
RRIDAB_2143838
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MEA1, also named Male-enhanced antigen 1, is a 185 amino acid protein, which is highly expressed in testis. MEA1 may play an important role in spermatogenesis and/or testis development. Endogenous protein was detected as two bands (34 kDa and 36 kDa). (PMID: 12444059)

Protocols

Product Specific Protocols
WB protocol for MEA1 antibody 10267-1-APDownload protocol
IHC protocol for MEA1 antibody 10267-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols