MCM8 Polyclonal antibody
MCM8 Polyclonal Antibody for WB, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IF, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 16451-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293T cells, NIH/3T3 cells |
| Positive IP detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 7 publications below |
| IF | See 1 publications below |
| IP | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
16451-1-AP targets MCM8 in WB, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | MCM8 fusion protein Ag8696 |
| Full Name | minichromosome maintenance complex component 8 |
| Calculated Molecular Weight | 880 aa, 98 kDa |
| Observed Molecular Weight | 90-95 kDa |
| GenBank Accession Number | BC008830 |
| Gene Symbol | MCM8 |
| Gene ID (NCBI) | 84515 |
| RRID | AB_2142833 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MCM8, also named as Minichromosome maintenance 8, is a 840 amino acid protein, which belongs to the MCM family. MCM8 is expressed highly in placenta, lung and pancreas and various tumors with highest levels in colon and lung cancers. MCM8 is a component of the MCM8-MCM9 complex, which involved in homologous recombination repair following DNA interstrand cross-links and plays a key role during gametogenesis. MCM8 exists some isoforms with MV 94 kDa and 70 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MCM8 antibody 16451-1-AP | Download protocol |
| IP protocol for MCM8 antibody 16451-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Commun DONSON and FANCM associate with different replisomes distinguished by replication timing and chromatin domain. | ||
Nat Commun MCM8IP activates the MCM8-9 helicase to promote DNA synthesis and homologous recombination upon DNA damage. | ||
Cell Death Dis Aberrantly expressed HORMAD1 disrupts nuclear localization of MCM8-MCM9 complex and compromises DNA mismatch repair in cancer cells. | ||
Nat Commun A multi-functional role for the MCM8/9 helicase complex in maintaining fork integrity during replication stress |
