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  • Featured Product
  • KD/KO Validated

MBD3 Polyclonal antibody

MBD3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 14258-1-AP

Synonyms

Methyl-CpG-binding protein MBD3, Methyl-CpG-binding domain protein 3, MBD 3

Validation Data Gallery

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  • Western Blot (WB) analysis of Y79 cells using MBD3 Polyclonal antibody (14258-1-AP)

    WB analysis of Y79 using 14258-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using MBD3 Polyclonal antibody (14258-1-AP)

    WB analysis of MCF-7 using 14258-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using MBD3 Polyclonal antibody (14258-1-AP)

    WB analysis of COLO 320 using 14258-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse testis tissue using MBD3 Polyclonal antibody (14258-1-AP)

    WB analysis of mouse testis using 14258-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using MBD3 Polyclonal antibody (14258-1-AP)

    WB analysis of Y79 using 14258-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using MBD3 Polyclonal antibody (14258-1-AP)

    WB analysis of MCF-7 using 14258-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using MBD3 Polyclonal antibody (14258-1-AP)

    WB analysis of MCF-7 using 14258-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using MBD3 Polyclonal antibody (14258-1-AP)

    WB analysis of Jurkat using 14258-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MBD3 Polyclonal antibody (14258-1-AP)

    WB analysis of HeLa using 14258-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using MBD3 Polyclonal antibody (14258-1-AP)

    WB analysis of Y79 using 14258-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using MBD3 Polyclonal antibody (14258-1-AP)

    WB analysis of HeLa using 14258-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14258-1-AP (MBD3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using MBD3 Polyclonal antibody (14258-1-AP)

    IP experiment of mouse testis using 14258-1-AP

    IP result of anti-MBD3 (IP:14258-1-AP, 4ug; Detection:14258-1-AP 1:800) with mouse testis tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using MBD3 Polyclonal antibody (14258-1-AP)

    IHC staining of human endometrial cancer using 14258-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 14258-1-AP (MBD3 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using MBD3 Polyclonal antibody (14258-1-AP)

    IHC staining of human endometrial cancer using 14258-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 14258-1-AP (MBD3 antibody) at dilution of 1:100 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MBD3 Polyclonal antibody (14258-1-AP)

    IF Staining of HepG2 using 14258-1-AP

    Immunofluorescent analysis of HepG2 cells, using MBD3 antibody 14258-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using MBD3 Polyclonal antibody (14258-1-AP)

    IF Staining of HepG2 using 14258-1-AP

    Immunofluorescent analysis of HepG2 cells, using MBD3 antibody 14258-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inY79 cells, COLO 320 cells, HeLa cells, Jurkat cells, MCF-7 cells, mouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14258-1-AP targets MBD3 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MBD3 fusion protein Ag5531
Full Name methyl-CpG binding domain protein 3
Calculated Molecular Weight 33 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC043619
Gene Symbol MBD3
Gene ID (NCBI) 53615
RRIDAB_2139745
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MBD3 does not bind DNA by itself. It recruits histone deacetylases and DNA methyltransferases. MBD3 acts as transcriptional repressor and plays a role in gene silencing.(PMID:9774669) .

Protocols

Product Specific Protocols
WB protocol for MBD3 antibody 14258-1-APDownload protocol
IHC protocol for MBD3 antibody 14258-1-APDownload protocol
IF protocol for MBD3 antibody 14258-1-APDownload protocol
IP protocol for MBD3 antibody 14258-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.

Authors - Xiao-Hui Wang
  • KD Validated
View Article

Dev Cell

Nucleoporin Seh1 controls murine neocortical development via transcriptional repression of p21 in neural stem cells

Authors - Wenxiu Dai
View Article
humanWB,IF

Proc Natl Acad Sci U S A

Compensatory functions of histone deacetylase 1 (HDAC1) and HDAC2 regulate transcription and apoptosis during mouse oocyte development.

Authors - Ma Pengpeng P
View Article
humanWB,IHC

Cancers (Basel)

MBD3 as a Potential Biomarker for Colon Cancer: Implications for Epithelial-Mesenchymal Transition (EMT) Pathways

Authors - Yuntao Ding
View Article
humanWB,ChIP

Oncol Lett

Demethylation of HACE1 gene promoter by propofol promotes autophagy of human A549 cells.

Authors - Shanshan Li
View Article
humanWB

Genet Test Mol Biomarkers

The Expression of MBD6 Is Associated with Tumor Size in Uterine Leiomyomas.

Authors - Mei-Rong Liang
View Article