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MAPK15 Polyclonal antibody

MAPK15 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13452-1-AP

Synonyms

ERK 7, ERK 8, ERK7, ERK8, MAP kinase 15, MAPK 15, MAPK15

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse lung tissue using MAPK15 Polyclonal antibody (13452-1-AP)

    WB analysis of mouse lung using 13452-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 13452-1-AP (MAPK15 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse lung tissue using MAPK15 Polyclonal antibody (13452-1-AP)

    IP experiment of mouse lung using 13452-1-AP

    IP result of anti-MAPK15 (IP:13452-1-AP, 4ug; Detection:13452-1-AP 1:500) with mouse lung tissue lysate 3800ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using MAPK15 Polyclonal antibody (13452-1-AP)

    IHC staining of human kidney using 13452-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13452-1-AP (MAPK15 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using MAPK15 Polyclonal antibody (13452-1-AP)

    IHC staining of human kidney using 13452-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13452-1-AP (MAPK15 antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inmouse lung tissue
Positive IP detected inmouse lung tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

13452-1-AP targets MAPK15 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAPK15 fusion protein Ag4255
Full Name mitogen-activated protein kinase 15
Calculated Molecular Weight 544 aa, 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC028034
Gene Symbol MAPK15
Gene ID (NCBI) 225689
RRIDAB_2877950
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MAPK15 antibody 13452-1-APDownload protocol
IHC protocol for MAPK15 antibody 13452-1-APDownload protocol
IP protocol for MAPK15 antibody 13452-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncol Lett

Metastasis-associated gene MAPK15 promotes the migration and invasion of osteosarcoma cells via the c-Jun/MMPs pathway.

Authors - Zexin Su
View Article