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LIN37 Polyclonal antibody

LIN37 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26651-1-AP

Synonyms

Antolefinin, F25965, lin 37, lin 37 homolog (C. elegans), LIN37, Protein lin 37 homolog, ZK418.4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using LIN37 Polyclonal antibody (26651-1-AP)

    WB analysis using 26651-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26651-1-AP (LIN37 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using LIN37 Polyclonal antibody (26651-1-AP)

    IF Staining of A549 using 26651-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using 26651-1-AP (LIN37 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inA549 cells, Jurkat cells
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26651-1-AP targets LIN37 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LIN37 fusion protein Ag24565
Full Name lin-37 homolog (C. elegans)
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC009071
Gene Symbol LIN37
Gene ID (NCBI) 55957
RRIDAB_2880590
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LIN37, also named as Antolefinin and MSTP064, is a component of the DREAM complex (also named LINC complex). LIN37 migrates to 37 kDa for modification.

Protocols

Product Specific Protocols
WB protocol for LIN37 antibody 26651-1-APDownload protocol
IF protocol for LIN37 antibody 26651-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols