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  • Featured Product
  • KD/KO Validated

LIMD2 Polyclonal antibody

LIMD2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15471-1-AP

Synonyms

LIM domain containing 2, LIMD2

Validation Data Gallery

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  • Western Blot (WB) analysis of U-937 cells using LIMD2 Polyclonal antibody (15471-1-AP)

    WB analysis of U-937 using 15471-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 15471-1-AP (LIMD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using LIMD2 Polyclonal antibody (15471-1-AP)

    WB analysis of Raji using 15471-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 15471-1-AP (LIMD2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LIMD2 Polyclonal antibody (15471-1-AP)

    IHC staining of human liver cancer using 15471-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 15471-1-AP (LIMD2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using LIMD2 Polyclonal antibody (15471-1-AP)

    IHC staining of human liver cancer using 15471-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 15471-1-AP (LIMD2 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using LIMD2 Polyclonal antibody (15471-1-AP)

    IHC staining of human brain using 15471-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15471-1-AP (LIMD2 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using LIMD2 Polyclonal antibody (15471-1-AP)

    IHC staining of human brain using 15471-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 15471-1-AP (LIMD2 antibody) at dilution of 1:50 (under 40x lens).

Tested Applications

Positive WB detected inU-937 cells, Raji cells
Positive IHC detected inhuman liver cancer tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15471-1-AP targets LIMD2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LIMD2 fusion protein Ag7775
Full Name LIM domain containing 2
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 16-20 kDa
GenBank Accession NumberBC004400
Gene Symbol LIMD2
Gene ID (NCBI) 80774
RRIDAB_10859817
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LIMD2 antibody 15471-1-APDownload protocol
IHC protocol for LIMD2 antibody 15471-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Bioengineered

LIM domain-containing 2 (LIMD2) promotes the progress of ovarian cancer via the focal adhesion signaling pathway.

Authors - Lixin Chen
View Article
humanWB

Mol Biol Rep

Knockdown of LIMD2 inhibits the progression of ovarian carcinoma through ERK1/2 pathway

Authors - Haiyang Hu
  • KD Validated
View Article