GYS1 AntibodyRabbit Polyclonal
|Tested applications||ELISA, WB, IHC, IF, FC|
|Cited applications||IF, WB|
|Species specificity||Human,Mouse,Rat; other species not tested.|
|Positive WB detected in||K-562 cells, HeLa cells|
|Positive IHC detected in||human prostate cancer tissue, human skeletal muscle tissue|
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Recommended dilution||WB: 1:200-1:2000|
IP = Immunoprecipitation
- K-562 cells were subjected to SDS PAGE followed by western blot with 10566-1-AP(GYS1 antibody) at dilution of 1:500
- Immunohistochemical of paraffin-embedded human prostate cancer using 10566-1-AP(GYS1 antibody) at dilution of 1:50 (under 25x lens)
- Immunofluorescent analysis of HepG2 cells, using GYS1 antibody 10566-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
- 1X10^6 HepG2 cells were stained with 0.2ug GYS1 antibody (10566-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.
|Source||Rabbit||Purification method||Antigen affinity purification|
|Isotype||IgG||Storage||PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.|
|Immunogen||GYS1 fusion protein ag0857||Full name||glycogen synthase 1 (muscle)|
|BC007688||Gene ID (NCBI)||2997|
|Gene symbol||GYS1||Synonyms||glycogen synthase 1 (muscle), GSY, GYS, GYS1|
GYS1(Glycogen [starch] synthase, muscle) is the the rate limiting enzyme of the insulin-induced glycogenesis, transferring glucose units from UDP-Glc to a glycogen primer. It catalyzes the linear addition of glucose residues to the branching structure of glycogen, providing a convenient store of glucose for times of metabolic need. This protein has 2 isoforms produced by alternative splicing.