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  • KD/KO Validated

GYS1 Polyclonal antibody

GYS1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 10566-1-AP

Synonyms

glycogen synthase 1 (muscle), GSY, GYS, GYS1



Tested Applications

Positive WB detected inHeLa cells, K-562 cells, Jurkat cells, mouse skeletal muscle tissue, rat heart tissue, rat skeletal muscle tissue
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman liver tissue, human skeletal muscle tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10566-1-AP targets GYS1 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GYS1 fusion protein Ag0857
Full Name glycogen synthase 1 (muscle)
Calculated Molecular Weight 84 kDa
Observed Molecular Weight 84 kDa
GenBank Accession NumberBC007688
Gene Symbol GYS1
Gene ID (NCBI) 2997
RRIDAB_2116401
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GYS1(Glycogen [starch] synthase, muscle) is the the rate limiting enzyme of the insulin-induced glycogenesis, transferring glucose units from UDP-Glc to a glycogen primer. It catalyzes the linear addition of glucose residues to the branching structure of glycogen, providing a convenient store of glucose for times of metabolic need. This protein has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for GYS1 antibody 10566-1-APDownload protocol
IHC protocol for GYS1 antibody 10566-1-APDownload protocol
IF protocol for GYS1 antibody 10566-1-APDownload protocol
IP protocol for GYS1 antibody 10566-1-APDownload protocol
FC protocol for GYS1 antibody 10566-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Biol Sci

PYGL-mediated glucose metabolism reprogramming promotes EMT phenotype and metastasis of pancreatic cancer

Authors - Qian Ji
humanWB,IHC

J Invest Dermatol

Enhanced Glycogen Metabolism Supports the Survival and Proliferation of HPV-Infected Keratinocytes in Condylomata Acuminata.

Authors - Zhichao Gu
  • KD Validated
mouseWB

Arterioscler Thromb Vasc Biol

Inducible metabolic adaptation promotes mesenchymal stem cell therapy for ischemia: a hypoxia-induced and glycogen-based energy prestorage strategy.

Authors - Hongming Zhu
ratWB

Metabolism

Recurrent hypoglycemia increases hepatic gluconeogenesis without affecting glycogen metabolism or systemic lipolysis in rat

Authors - Zejian Liu
mouseWB

Mol Nutr Food Res

Douchi Peptides Vy and Sfllr Improve Glucose Homeostasis and Gut Dysbacteriosis In High-Fat Diet-Induced Insulin Resistant Mice

Authors - Songfeng Yu
mouseWB,IF

J Lipid Res

Dynamic and differential regulation of proteins that coat lipid droplets in fatty liver dystrophic mice.

Authors - Hall Angela M AM