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GTF2A2 Polyclonal antibody

GTF2A2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10540-1-AP

Synonyms

TFIIA-12, TFIIA gamma, TFIIA 12, TFIIA, TF2A2

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using GTF2A2 Polyclonal antibody (10540-1-AP)

    WB analysis of mouse testis using 10540-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 10540-1-AP (GTF2A2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GTF2A2 Polyclonal antibody (10540-1-AP)

    WB analysis of HeLa using 10540-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10540-1-AP (GTF2A2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using GTF2A2 Polyclonal antibody (10540-1-AP)

    IHC staining of mouse liver using 10540-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10540-1-AP (GTF2A2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using GTF2A2 Polyclonal antibody (10540-1-AP)

    IHC staining of mouse liver using 10540-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10540-1-AP (GTF2A2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat liver tissue using GTF2A2 Polyclonal antibody (10540-1-AP)

    IHC staining of rat liver using 10540-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 10540-1-AP (GTF2A2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat liver tissue using GTF2A2 Polyclonal antibody (10540-1-AP)

    IHC staining of rat liver using 10540-1-AP

    Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 10540-1-AP (GTF2A2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using GTF2A2 Polyclonal antibody (10540-1-AP)

    IHC staining of mouse heart using 10540-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10540-1-AP (GTF2A2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using GTF2A2 Polyclonal antibody (10540-1-AP)

    IHC staining of mouse heart using 10540-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 10540-1-AP (GTF2A2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse testis tissue, HeLa cells
Positive IHC detected inmouse liver tissue, mouse heart tissue, rat liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10540-1-AP targets GTF2A2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GTF2A2 fusion protein Ag0825
Full Name general transcription factor IIA, 2, 12kDa
Calculated Molecular Weight 12 kDa
Observed Molecular Weight 12 kDa
GenBank Accession NumberBC001919
Gene Symbol GTF2A2
Gene ID (NCBI) 2958
RRIDAB_2114351
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transcription mediated by RNA polymerase II depends on a set of different transcription factors to form the pre-initiation complex. TFIIA is involved in the construction of this complex and increases the affinity of TBP for the DNA union region [PMID:20480367]. TFIIA in a complex with TBP mediates transcriptional activity [PMID: 11030333]. GTF2A2 is one subunit of TFIIA.

Protocols

Product Specific Protocols
WB protocol for GTF2A2 antibody 10540-1-APDownload protocol
IHC protocol for GTF2A2 antibody 10540-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols