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GEFT Monoclonal antibody

GEFT Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

5A11F8

Cat no : 66012-1-Ig

Synonyms

ARHGEF25, RhoA/RAC/CDC42 exchange factor, Rho guanine nucleotide exchange factor 25, p63RhoGEF

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skeletal muscle tissue using GEFT Monoclonal antibody (66012-1-Ig)

    WB analysis of mouse skeletal muscle using 66012-1-Ig

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using GEFT Monoclonal antibody (66012-1-Ig)

    WB analysis of human brain using 66012-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using GEFT Monoclonal antibody (66012-1-Ig)

    WB analysis of mouse brain using 66012-1-Ig

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat brain tissue using GEFT Monoclonal antibody (66012-1-Ig)

    WB analysis of rat brain using 66012-1-Ig

    rat brain tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat skeletal muscle tissue using GEFT Monoclonal antibody (66012-1-Ig)

    WB analysis of rat skeletal muscle using 66012-1-Ig

    rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using GEFT Monoclonal antibody (66012-1-Ig)

    WB analysis of human brain using 66012-1-Ig

    human brain tissue were subjected to SDS PAGE followed by western blot with 66012-1-Ig (GEFT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using GEFT Monoclonal antibody (66012-1-Ig)

    IHC staining of human heart using 66012-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart using 66012-1-Ig(GEFT antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using GEFT Monoclonal antibody (66012-1-Ig)

    IHC staining of human heart using 66012-1-Ig

    Immunohistochemical analysis of paraffin-embedded human heart using 66012-1-Ig(GEFT antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using GEFT Monoclonal antibody (66012-1-Ig)

    IHC staining of human brain using 66012-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain using 66012-1-Ig(GEFT antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using GEFT Monoclonal antibody (66012-1-Ig)

    IHC staining of human brain using 66012-1-Ig

    Immunohistochemical analysis of paraffin-embedded human brain using 66012-1-Ig(GEFT antibody) at dilution of 1:50 (under 40x lens).

  • Flow cytometry (FC) experiment of HEK-293 cells using GEFT Monoclonal antibody (66012-1-Ig)

    FC experiment of HEK-293 using 66012-1-Ig

    1x10^6 HEK-293 cells were intracellularly stained with 0.8 ug GEFT Monoclonal antibody (66012-1-Ig, Clone:5A11F8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG2a isotype control Mouse McAb (66360-2-Ig, Clone: 11A1B2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inmouse skeletal muscle tissue, human brain tissue, mouse brain tissue, rat brain tissue, rat skeletal muscle tissue
Positive IHC detected inhuman heart tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

66012-1-Ig targets GEFT in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Reactivity human, mouse, rat, pig, rabbit
Cited Reactivityhuman
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen GEFT fusion protein Ag10819
Full Name RhoA/RAC/CDC42 exchange factor
Calculated Molecular Weight64 kDa
Observed Molecular Weight 53 kDa, 63 kDa
GenBank Accession NumberBC012860
Gene Symbol GEFT
Gene ID (NCBI) 115557
RRIDAB_11044192
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GEFT, also named as p63RhoGEF, may play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. GETF links specifically G alpha q/11-coupled receptors to RHOA activation. GEFT may be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. It is involved in skeletal myogenesis. GEFT is expressed as three isoforms (64, 53 and 68kDa).

Protocols

Product Specific Protocols
WB protocol for GEFT antibody 66012-1-IgDownload protocol
IHC protocol for GEFT antibody 66012-1-IgDownload protocol
FC protocol for GEFT antibody 66012-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cancer Sci

Inhibition of heat shock protein 90 destabilizes receptor tyrosine kinase ROR1 in lung adenocarcinoma.

Authors - Behnoush Khaledian
View Article