• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Filter:
  • Western Blot (WB) analysis of Raji cells using GCET2 Polyclonal antibody (13447-1-AP)

    WB analysis of Raji using 13447-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 13447-1-AP (GCET2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using GCET2 Polyclonal antibody (13447-1-AP)

    IF Staining of Raji using 13447-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using GCET2 antibody (13447-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

GCET2 Polyclonal antibody

GCET2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 13447-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Germinal center-associated signaling and motility protein, Germinal center-associated lymphoma protein, Germinal center B-cell-expressed transcript 2 protein, GCSAM, GCAT2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Please visit your regions distributor:

Tested Applications

Positive WB detected inRaji cells
Positive IF/ICC detected inRaji cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13447-1-AP targets GCET2 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GCET2 fusion protein Ag4280 Predict reactive species
Full Name germinal center expressed transcript 2
Calculated Molecular Weight 178 aa, 21 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC030506
Gene Symbol GCET2
Gene ID (NCBI) 257144
RRIDAB_2877949
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N6F7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GCET2, also named HGAL, is a newly gene that has been shown to be a useful marker for germinal centre (GC) B cells and GC B-cell derived malignancies, including follicular lymphomas and germinal centre B cell-like diffuse large B-cell lymphomas (GCB-DLBCLs), and is a useful prognosticator for DLBCLs. GCET2 may be an adaptor protein in GC B cells that transduces signals from GC B-cell membrane to the cytosol via its association with GRB2.

Protocols

Product Specific Protocols
WB protocol for GCET2 antibody 13447-1-APDownload protocol
IF protocol for GCET2 antibody 13447-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols