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GBX2 Polyclonal antibody

GBX2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, chIP, ELISA

Conjugate

Unconjugated

Cat no : 21639-1-AP

Synonyms

Homeobox protein GBX-2, Gastrulation and brain-specific homeobox protein 2

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using GBX2 Polyclonal antibody (21639-1-AP)

    WB analysis of HeLa using 21639-1-AP

    WB result of GBX2 antibody (21639-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-GBX2 transfected HeLa cells.

  • Western Blot (WB) analysis of mouse stomach tissue using GBX2 Polyclonal antibody (21639-1-AP)

    WB analysis of mouse stomach using 21639-1-AP

    mouse stomach tissue were subjected to SDS PAGE followed by western blot with 21639-1-AP (GBX2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using GBX2 Polyclonal antibody (21639-1-AP)

    IP experiment of HeLa using 21639-1-AP

    IP result of anti-GBX2 (IP:21639-1-AP, 4ug; Detection:21639-1-AP 1:300) with HeLa cells lysate 3000ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GBX2 Polyclonal antibody (21639-1-AP)

    IF Staining of HeLa using 21639-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GBX2 antibody (21639-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using GBX2 Polyclonal antibody (21639-1-AP)

    IF Staining of HeLa using 21639-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 21639-1-AP (GBX2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inmouse stomach tissue, HeLa cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21639-1-AP targets GBX2 in WB, IF/ICC, IP, chIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GBX2 fusion protein Ag16288
Full Name gastrulation brain homeobox 2
Calculated Molecular Weight 348 aa, 37 kDa
Observed Molecular Weight 33-37 kDa
GenBank Accession NumberBC137448
Gene Symbol GBX2
Gene ID (NCBI) 2637
RRIDAB_2878896
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Homeobox protein GBX-2 (GBX-2) is a homeobox gene essential for normal development of the midbrain and the anterior hindbrain. It mainly expressed in the brain, and located in nucleoplasm. It is presumably a transcription factor that is likely to regulate the expression of genes involved in establishing early A/P pattern in the neural plate (PMID: 9247335).

Protocols

Product Specific Protocols
WB protocol for GBX2 antibody 21639-1-APDownload protocol
IF protocol for GBX2 antibody 21639-1-APDownload protocol
IP protocol for GBX2 antibody 21639-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell Stem Cell

Thalamocortical organoids enable in vitro modeling of 22q11.2 microdeletion associated with neuropsychiatric disorders

Authors - David Shin
View Article
humanchIP

RNA Biol

NCBP3/SNHG6 inhibits GBX2 transcription in a histone modification manner to facilitate the malignant biological behaviour of glioma cells.

Authors - Xiwen Li
View Article
humanWB,ChIP

Funct Integr Genomics

Oncogenic GBX2 promotes the malignant behaviors of bladder cancer cells by binding to the ITGA5 promoter and activating its transcription.

Authors - Yaoyao Xiong
View Article