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GAGE2D Polyclonal antibody

GAGE2D Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12532-1-AP

Synonyms

GAGE-2D, GAGE 8, G antigen 8, G antigen 2D, CT4.8

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using GAGE2D Polyclonal antibody (12532-1-AP)

    WB analysis of K-562 using 12532-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 12532-1-AP (GAGE2D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using GAGE2D Polyclonal antibody (12532-1-AP)

    WB analysis of human testis using 12532-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 12532-1-AP (GAGE2D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using GAGE2D Polyclonal antibody (12532-1-AP)

    WB analysis of HeLa using 12532-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12532-1-AP (GAGE2D antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GAGE2D Polyclonal antibody (12532-1-AP)

    IHC staining of human liver cancer using 12532-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12532-1-AP (GAGE2D Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using GAGE2D Polyclonal antibody (12532-1-AP)

    IHC staining of human liver cancer using 12532-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12532-1-AP (GAGE2D Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using GAGE2D Polyclonal antibody (12532-1-AP)

    IHC staining of human testis using 12532-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12532-1-AP (GAGE2D Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using GAGE2D Polyclonal antibody (12532-1-AP)

    IHC staining of human testis using 12532-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 12532-1-AP (GAGE2D Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using GAGE2D Polyclonal antibody (12532-1-AP)

    IF Staining of HepG2 using 12532-1-AP

    Immunofluorescent analysis of HepG2 cells, using GAGE2D antibody 12532-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inK-562 cells, HeLa cells, human testis tissue
Positive IHC detected inhuman liver cancer tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12532-1-AP targets GAGE2D in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen GAGE2D fusion protein Ag3230
Full Name G antigen 2D
Calculated Molecular Weight 116 aa, 13 kDa
Observed Molecular Weight 18-22 kDa
GenBank Accession NumberBC018052
Gene Symbol GAGE2D
Gene ID (NCBI) 729408
RRIDAB_2877864
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GAGE2D, also named as GAGE8 and CT4.8, is expressed in testis and tumor tissues.

Protocols

Product Specific Protocols
WB protocol for GAGE2D antibody 12532-1-APDownload protocol
IHC protocol for GAGE2D antibody 12532-1-APDownload protocol
IF protocol for GAGE2D antibody 12532-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols