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  • Featured Product
  • KD/KO Validated

EIF2B1 Polyclonal antibody

EIF2B1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 18010-1-AP

Synonyms

Translation initiation factor eIF2B subunit alpha, EIF2BA, eIF2B GDP-GTP exchange factor subunit alpha, EIF2B, EIF 2Balpha

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using EIF2B1 Polyclonal antibody (18010-1-AP)

    WB analysis of MCF-7 using 18010-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 18010-1-AP (EIF2B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using EIF2B1 Polyclonal antibody (18010-1-AP)

    WB analysis of K-562 using 18010-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 18010-1-AP (EIF2B1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using EIF2B1 Polyclonal antibody (18010-1-AP)

    WB analysis of HeLa using 18010-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18010-1-AP (EIF2B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of K-562 cells using EIF2B1 Polyclonal antibody (18010-1-AP)

    IP experiment of K-562 using 18010-1-AP

    IP result of anti-EIF2B1 (IP:18010-1-AP, 4ug; Detection:18010-1-AP 1:700) with K-562 cells lysate 3200ug.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using EIF2B1 Polyclonal antibody (18010-1-AP)

    IHC staining of mouse brain using 18010-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18010-1-AP (EIF2B1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EIF2B1 Polyclonal antibody (18010-1-AP)

    IHC staining of human breast cancer using 18010-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18010-1-AP (EIF2B1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EIF2B1 Polyclonal antibody (18010-1-AP)

    IHC staining of human breast cancer using 18010-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18010-1-AP (EIF2B1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using EIF2B1 Polyclonal antibody (18010-1-AP)

    IHC staining of mouse brain using 18010-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18010-1-AP (EIF2B1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using EIF2B1 Polyclonal antibody (18010-1-AP)

    IHC staining of mouse brain using 18010-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18010-1-AP (EIF2B1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using EIF2B1 Polyclonal antibody (18010-1-AP)

    IHC staining of mouse brain using 18010-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18010-1-AP (EIF2B1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EIF2B1 Polyclonal antibody (18010-1-AP)

    IF Staining of HeLa using 18010-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EIF2B1 antibody (18010-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Tested Applications

Positive WB detected inMCF7 cells, HeLa cells, K-562 cells
Positive IP detected inK-562 cells
Positive IHC detected inmouse brain tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18010-1-AP targets EIF2B1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EIF2B1 fusion protein Ag12509
Full Name eukaryotic translation initiation factor 2B, subunit 1 alpha, 26kDa
Calculated Molecular Weight 305 aa, 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC103763
Gene Symbol EIF2B1
Gene ID (NCBI) 1967
RRIDAB_2261996
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for EIF2B1 antibody 18010-1-APDownload protocol
IHC protocol for EIF2B1 antibody 18010-1-APDownload protocol
IF protocol for EIF2B1 antibody 18010-1-APDownload protocol
IP protocol for EIF2B1 antibody 18010-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,CoIP

Nat Microbiol

Inhibition of the integrated stress response by viral proteins that block p-eIF2-eIF2B association.

Authors - Huib H Rabouw
View Article
humanWB

Nat Commun

Sugar phosphate activation of the stress sensor eIF2B.

Authors - Qi Hao
View Article
humanWB

Elife

Pharmacological dimerization and activation of the exchange factor eIF2B antagonizes the integrated stress response.

Authors - Carmela Sidrauski
View Article
WB

Elife

The small molecule ISRIB rescues the stability and activity of Vanishing White Matter Disease eIF2B mutant complexes.

Authors - Yao Liang Wong
View Article
humanWB

Elife

eIF2B conformation and assembly state regulate the integrated stress response.

Authors - Michael Schoof
View Article
human, mouseWB

FASEB J

Analysis of the subunit organization of the eIF2B complex reveals new insights into its structure and regulation.

Authors - Noel C Wortham
View Article