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EFS Polyclonal antibody

EFS Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 20538-1-AP

Synonyms

CAS3, CASS3, EFS, EFS1, EFS2, HEFS, SIN

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EFS Polyclonal antibody (20538-1-AP)

    WB analysis using 20538-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 20538-1-AP (EFS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using EFS Polyclonal antibody (20538-1-AP)

    WB analysis of human placenta using 20538-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 20538-1-AP (EFS antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse thymus tissue using EFS Polyclonal antibody (20538-1-AP)

    IP experiment of mouse thymus using 20538-1-AP

    IP result of anti-EFS (IP:20538-1-AP, 4ug; Detection:20538-1-AP 1:300) with mouse thymus tissue lysate 3200ug.

  • Immunoprecipitation (IP) experiment of mouse thymus tissue using EFS Polyclonal antibody (20538-1-AP)

    IP experiment of mouse thymus using 20538-1-AP

    IP result of anti-EFS (IP:20538-1-AP, 4ug; Detection:20538-1-AP 1:300) with mouse thymus tissue lysate 2800ug.

  • Immunohistochemistry (IHC) staining of human spleen tissue using EFS Polyclonal antibody (20538-1-AP)

    IHC staining of human spleen using 20538-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 20538-1-AP (EFS antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human spleen tissue using EFS Polyclonal antibody (20538-1-AP)

    IHC staining of human spleen using 20538-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 20538-1-AP (EFS antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using EFS Polyclonal antibody (20538-1-AP)

    IF Staining of HepG2 using 20538-1-AP

    Immunofluorescent analysis of HepG2 cells, using EFS antibody 20538-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

Tested Applications

Positive WB detected inmouse thymus tissue, human placenta tissue
Positive IP detected inmouse thymus tissue
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

20538-1-AP targets EFS in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen EFS fusion protein Ag14565
Full Name embryonal Fyn-associated substrate
Calculated Molecular Weight 561 aa, 59 kDa
Observed Molecular Weight 80-90 kDa, 67-70 kDa
GenBank Accession NumberBC034246
Gene Symbol EFS
Gene ID (NCBI) 10278
RRIDAB_10695758
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for EFS antibody 20538-1-APDownload protocol
IHC protocol for EFS antibody 20538-1-APDownload protocol
IF protocol for EFS antibody 20538-1-APDownload protocol
IP protocol for EFS antibody 20538-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Genet

LYPD3, a New Biomarker and Therapeutic Target for Acute Myelogenous Leukemia.

Authors - Tingting Hu
View Article