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DIXDC1 Polyclonal antibody

DIXDC1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13816-1-AP

Synonyms

CCD1, Coiled coil DIX1, Coiled coil protein DIX1, DIX domain containing 1, DIXDC1, Dixin, KIAA1735

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skeletal muscle tissue using DIXDC1 Polyclonal antibody (13816-1-AP)

    WB analysis of mouse skeletal muscle using 13816-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13816-1-AP (DIXDC1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using DIXDC1 Polyclonal antibody (13816-1-AP)

    WB analysis of mouse heart using 13816-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 13816-1-AP (DIXDC1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human heart tissue using DIXDC1 Polyclonal antibody (13816-1-AP)

    IHC staining of human heart using 13816-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13816-1-AP (DIXDC1 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human heart tissue using DIXDC1 Polyclonal antibody (13816-1-AP)

    IHC staining of human heart using 13816-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13816-1-AP (DIXDC1 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using DIXDC1 Polyclonal antibody (13816-1-AP)

    IHC staining of human kidney using 13816-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 13816-1-AP (DIXDC1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using DIXDC1 Polyclonal antibody (13816-1-AP)

    IHC staining of human kidney using 13816-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 13816-1-AP (DIXDC1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using DIXDC1 Polyclonal antibody (13816-1-AP)

    IHC staining of human testis using 13816-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 13816-1-AP (DIXDC1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using DIXDC1 Polyclonal antibody (13816-1-AP)

    IHC staining of human testis using 13816-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 13816-1-AP (DIXDC1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using DIXDC1 Polyclonal antibody (13816-1-AP)

    IHC staining of human skin using 13816-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 13816-1-AP (DIXDC1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using DIXDC1 Polyclonal antibody (13816-1-AP)

    IHC staining of human skin using 13816-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 13816-1-AP (DIXDC1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using DIXDC1 Polyclonal antibody (13816-1-AP)

    IHC staining of human lung using 13816-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 13816-1-AP (DIXDC1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using DIXDC1 Polyclonal antibody (13816-1-AP)

    IHC staining of human lung using 13816-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 13816-1-AP (DIXDC1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using DIXDC1 Polyclonal antibody (13816-1-AP)

    IHC staining of human ovary using 13816-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 13816-1-AP (DIXDC1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using DIXDC1 Polyclonal antibody (13816-1-AP)

    IHC staining of human ovary using 13816-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 13816-1-AP (DIXDC1 antibody) at dilution of 1:100 (under 40x lens).

Tested Applications

Positive WB detected inmouse skeletal muscle tissue, mouse heart tissue
Positive IHC detected inhuman heart tissue, human kidney tissue, human testis tissue, human skin tissue, human lung tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

13816-1-AP targets DIXDC1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen DIXDC1 fusion protein Ag4904
Full Name DIX domain containing 1
Calculated Molecular Weight 683 aa, 77 kDa
Observed Molecular Weight 70-75 kDa
GenBank Accession NumberBC033034
Gene Symbol DIXDC1
Gene ID (NCBI) 85458
RRIDAB_2091188
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for DIXDC1 antibody 13816-1-APDownload protocol
IHC protocol for DIXDC1 antibody 13816-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Mol Neurobiol

Wnt Signaling Pathways Are Dysregulated in Rat Female Cerebellum Following Early Methyl Donor Deficiency.

Authors - Jérèmy Willekens
View Article
humanWB

Mol Carcinog

The oncogenic role and regulatory mechanism of ACAA2 in human ovarian cancer

Authors - Yahui Leng
View Article