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D2HGDH Monoclonal antibody

D2HGDH Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1E9B3

Cat no : 66364-1-Ig

Synonyms

EC:1.1.99.39, D-2-hydroxyglutarate dehydrogenase, mitochondrial, D2HGD, 1E9B3

Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using D2HGDH Monoclonal antibody (66364-1-Ig)

    WB analysis of NIH/3T3 using 66364-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66364-1-Ig (D2HGDH Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of C6 cells using D2HGDH Monoclonal antibody (66364-1-Ig)

    WB analysis of C6 using 66364-1-Ig

    C6 cells were subjected to SDS PAGE followed by western blot with 66364-1-Ig (D2HGDH Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat heart tissue using D2HGDH Monoclonal antibody (66364-1-Ig)

    WB analysis of rat heart using 66364-1-Ig

    rat heart tissue were subjected to SDS PAGE followed by western blot with 66364-1-Ig (D2HGDH Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using D2HGDH Monoclonal antibody (66364-1-Ig)

    IHC staining of human liver cancer using 66364-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66364-1-Ig (D2HGDH Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using D2HGDH Monoclonal antibody (66364-1-Ig)

    IHC staining of human liver cancer using 66364-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66364-1-Ig (D2HGDH Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using D2HGDH Monoclonal antibody (66364-1-Ig)

    IF Staining of HepG2 using 66364-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using D2HGDH antibody (66364-1-Ig, Clone: 1E9B3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using D2HGDH Monoclonal antibody (66364-1-Ig)

    IF Staining of HepG2 using 66364-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using D2HGDH antibody (66364-1-Ig, Clone: 1E9B3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of HepG2 cells using D2HGDH Monoclonal antibody (66364-1-Ig)

    FC experiment of HepG2 using 66364-1-Ig

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human D2HGDH (66364-1-Ig, Clone:1E9B3) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive WB detected inNIH/3T3 cells, C6 cells, rat heart tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66364-1-Ig targets D2HGDH in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen D2HGDH fusion protein Ag5036
Full Name D-2-hydroxyglutarate dehydrogenase
Calculated Molecular Weight 521 aa, 56 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC036604
Gene Symbol D2HGDH
Gene ID (NCBI) 728294
RRIDAB_2881744
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

D2HGDH(D-2-hydroxyglutarate dehydrogenase, mitochondrial) is also named as D2HGD and belongs to the FAD-binding oxidoreductase/transferase type 4 family.It catalyzes the oxidation of D-2-hydroxyglutarate to alpha-ketoglutarate.Defects in D2HGDH are the cause of D-2-hydroxyglutaric aciduria type 1 (D2HGA1).

Protocols

Product Specific Protocols
WB protocol for D2HGDH antibody 66364-1-IgDownload protocol
IHC protocol for D2HGDH antibody 66364-1-IgDownload protocol
IF protocol for D2HGDH antibody 66364-1-IgDownload protocol
FC protocol for D2HGDH antibody 66364-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols