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CoraLite®594-conjugated D2HGDH Monoclonal antibody

D2HGDH Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E9B3

Cat no : CL594-66364

Synonyms

D2HGD, D2HGDH, FLJ42195

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite®594-conjugated D2HGDH Monoclonal antibody (CL594-66364)

    IF Staining of HepG2 using CL594-66364

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite®®594 D2HGDH antibody (CL594-66364, Clone: 1E9B3 ) at dilution of 1:100. DAPI (blue).

Tested Applications

Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66364 targets D2HGDH in IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen D2HGDH fusion protein Ag5036
Full Name D-2-hydroxyglutarate dehydrogenase
Calculated Molecular Weight 521 aa, 56 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC036604
Gene Symbol D2HGDH
Gene ID (NCBI) 728294
RRIDAB_2919981
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

D2HGDH(D-2-hydroxyglutarate dehydrogenase, mitochondrial) is also named as D2HGD and belongs to the FAD-binding oxidoreductase/transferase type 4 family.It catalyzes the oxidation of D-2-hydroxyglutarate to alpha-ketoglutarate.Defects in D2HGDH are the cause of D-2-hydroxyglutaric aciduria type 1 (D2HGA1).

Protocols

Product Specific Protocols
IF protocol for CL594 D2HGDH antibody CL594-66364Download protocol
Standard Protocols
Click here to view our Standard Protocols