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CHTF18 Polyclonal antibody

CHTF18 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13030-1-AP

Synonyms

C16orf41, C321D2.2, C321D2.3, C321D2.4, CHL12, CHTF18, Ctf18, hCTF18, RUVBL

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHTF18 Polyclonal antibody (13030-1-AP)

    WB analysis using 13030-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13030-1-AP (CHTF18 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using CHTF18 Polyclonal antibody (13030-1-AP)

    WB analysis of Y79 using 13030-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 13030-1-AP (CHTF18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human medulloblastoma tissue using CHTF18 Polyclonal antibody (13030-1-AP)

    IHC staining of human medulloblastoma using 13030-1-AP

    Immunohistochemical analysis of paraffin-embedded human medulloblastoma using 13030-1-AP (CHTF18 antibody) at dilution of 1:100 (under 10x lens).

Tested Applications

Positive WB detected inHeLa cells, Y79 cells
Positive IHC detected inhuman medulloblastoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13030-1-AP targets CHTF18 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHTF18 fusion protein Ag3672
Full Name CTF18, chromosome transmission fidelity factor 18 homolog (S. cerevisiae)
Calculated Molecular Weight 1184 aa, 129 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC018184
Gene Symbol CHTF18
Gene ID (NCBI) 63922
RRIDAB_2260469
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CHTF18 antibody 13030-1-APDownload protocol
IHC protocol for CHTF18 antibody 13030-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols