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CDC14A Polyclonal antibody

CDC14A Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 13660-1-AP

Synonyms

cdc14, CDC14A, hCDC14

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of human heart tissue using CDC14A Polyclonal antibody (13660-1-AP)

    WB analysis of human heart using 13660-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 13660-1-AP (CDC14A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using CDC14A Polyclonal antibody (13660-1-AP)

    WB analysis of COLO 320 using 13660-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 13660-1-AP (CDC14A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using CDC14A Polyclonal antibody (13660-1-AP)

    WB analysis of human brain using 13660-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 13660-1-AP (CDC14A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CDC14A Polyclonal antibody (13660-1-AP)

    WB analysis of HeLa using 13660-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 13660-1-AP (CDC14A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inhuman heart tissue, human brain tissue, HeLa cells, COLO 320 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

13660-1-AP targets CDC14A in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CDC14A fusion protein Ag4556
Full Name CDC14 cell division cycle 14 homolog A (S. cerevisiae)
Calculated Molecular Weight 594 aa, 66 kDa
Observed Molecular Weight 66 kDa
GenBank Accession NumberBC038979
Gene Symbol CDC14A
Gene ID (NCBI) 8556
RRIDAB_2260218
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for CDC14A antibody 13660-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Mol Med Rep

Phospho‑regulation of Cdc14A by polo‑like kinase 1 is involved in β‑cell function and cell cycle regulation.

Authors - Haiying Hu
View Article