Tested Applications
| Positive WB detected in | HEK-293 cells, A431 cells, PC-3 cells |
| Positive IHC detected in | human tonsillitis tissue, human placenta tissue, human prostate cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human placenta tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunohistochemistry (IHC) | IHC : 1:2500-1:10000 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IHC | See 6 publications below |
| IF | See 2 publications below |
| IP | See 1 publications below |
| FC | See 1 publications below |
| CoIP | See 2 publications below |
Product Information
66481-1-Ig targets B7-H3/CD276 in WB, IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, pig samples.
| Tested Reactivity | human, pig |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | B7-H3/CD276 fusion protein Ag6006 Predict reactive species |
| Full Name | CD276 molecule |
| Calculated Molecular Weight | 57 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC062581 |
| Gene Symbol | CD276 |
| Gene ID (NCBI) | 80381 |
| RRID | AB_2881847 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q5ZPR3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
B7-H3 (CD276) is a type I transmembrane protein expressed on many tissues and cell types. B7-H3 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for B7-H3/CD276 antibody 66481-1-Ig | Download protocol |
| IHC protocol for B7-H3/CD276 antibody 66481-1-Ig | Download protocol |
| IF protocol for B7-H3/CD276 antibody 66481-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Immunother Cancer Genome-scale CRISPR-Cas9 screen reveals novel regulators of B7-H3 in tumor cells. | ||
J Transl Med An N-glycoproteomic site-mapping analysis reveals glycoprotein alterations in esophageal squamous cell carcinoma. | ||
J Cancer Res Clin Oncol B7-H3 and CD47 co-expression in gastric cancer is a predictor of poor prognosis and potential targets for future dual-targeting immunotherapy | ||
J Cancer B7-H3 inhibits apoptosis of gastric cancer cell by interacting with Fibronectin. | ||
Int J Gen Med Comprehensive Analysis Reveals Distinct Immunological and Prognostic Characteristics of CD276/B7-H3 in Pan-Cancer | ||
FEBS Open Bio Fibronectin enhances tumor metastasis through B7-H3 in clear cell renal cell carcinoma |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Margarita (Verified Customer) (11-20-2024) | Secondary antibody: AF 488
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