CoraLite®594-conjugated B7-H3/CD276 Monoclonal antibody

• Featured Product
• KD/KO Validated

B7-H3/CD276 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Pig

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1E7D1

Cat no : CL594-66481

Synonyms

4Ig B7 H3, B7 H3, B7 homolog 3, B7H3, B7-H3, CD276, CD276 antigen, CD276 molecule, Costimulatory molecule, UNQ309/PRO352

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Validation Data Gallery

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  IF Staining of human tonsillitis using CL594-66481

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594-conjugated B7-H3/CD276 antibody (CL594-66481, Clone: 1E7D1 ) at dilution of 1:400.

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Tested Applications

• Positive IF detected in: human tonsillitis tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66481 targets B7-H3/CD276 in IF applications and shows reactivity with Human, Pig samples.

• Tested Reactivity: Human, Pig
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: B7-H3/CD276 fusion protein Ag6006
• Full Name: CD276 molecule
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC062581
• Gene Symbol: CD276
• Gene ID (NCBI): 80381
• RRID: AB_2883588
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

B7-H3 (CD276) is a type I transmembrane protein expressed on many tissues and cell types. B7-H3 is a 100-kDa glycoprotein that belongs to the B7 immunoregulatory family and participates in the regulation of T-cell-mediated immune response probably by functioning as both a T cell costimulator and coinhibitor (PMID: 25567370; 20696859). Overexpressed on a wide range of human solid cancers, B7-H3 has been implicated in cancer progression and metastasis and becomes an attractive target for cancer immunotherapy (PMID: 27208063).