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  • Featured Product
  • KD/KO Validated

CEP85L Polyclonal antibody

CEP85L Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 24588-1-AP

Synonyms

C6orf204, Serologically defined breast cancer antigen NY-BR-15, NY-BR-15, NY BR 15, Centrosomal protein of 85 kDa-like

Validation Data Gallery

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  • Western Blot (WB) analysis of PC-13 cells using CEP85L Polyclonal antibody (24588-1-AP)

    WB analysis of PC-13 using 24588-1-AP

    PC-13 cells were subjected to SDS PAGE followed by western blot with 24588-1-AP (C6orf204 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using CEP85L Polyclonal antibody (24588-1-AP)

    WB analysis of K-562 using 24588-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 24588-1-AP (CEP85L antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CEP85L Polyclonal antibody (24588-1-AP)

    IHC staining of mouse testis using 24588-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24588-1-AP (CEP85L antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inPC-13 cells
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:300-1:800
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24588-1-AP targets CEP85L in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CEP85L fusion protein Ag20235
Full Name chromosome 6 open reading frame 204
Calculated Molecular Weight 805 aa, 92 kDa
Observed Molecular Weight 56 kDa
GenBank Accession NumberBC153054
Gene Symbol CEP85L
Gene ID (NCBI) 387119
RRIDAB_2819012
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CEP85L, also named as C60rf204, is a gene which encodes a protein that has identified as a breast cancer antigen. Nothing more is known of its function at this time. There're five transcript variants with MW 45 kDa, 55-56 kDa and 91-92 kDa.

Protocols

Product Specific Protocols
WB protocol for CEP85L antibody 24588-1-APDownload protocol
IHC protocol for CEP85L antibody 24588-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Neuron

Pathogenic Variants in CEP85L Cause Sporadic and Familial Posterior Predominant Lissencephaly.

Authors - Meng-Han Tsai
View Article
humanWB,IF,CoIP

Neuron

Posterior Neocortex-Specific Regulation of Neuronal Migration by CEP85L Identifies Maternal Centriole-Dependent Activation of CDK5.

Authors - Andrew Kodani
  • KD Validated
View Article