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  • Featured Product
  • KD/KO Validated

C20orf3/APMAP Polyclonal antibody

C20orf3/APMAP Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Cat no : 25953-1-AP

Synonyms

C20orf3, UNQ1869/PRO4305, Protein BSCv, BSCv, APMAP

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using C20orf3/APMAP Polyclonal antibody (25953-1-AP)

    WB analysis of HepG2 using 25953-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25953-1-AP (C20orf3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using C20orf3/APMAP Polyclonal antibody (25953-1-AP)

    IP experiment of mouse liver using 25953-1-AP

    IP result of anti-C20orf3/APMAP (IP:25953-1-AP, 4ug; Detection:25953-1-AP 1:300) with mouse liver tissue lysate 4800ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using C20orf3/APMAP Polyclonal antibody (25953-1-AP)

    IHC staining of human kidney using 25953-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25953-1-AP (C20orf3 Antibody) at dilution of 1:200 (under 40x lens).

Tested Applications

Positive WB detected inHepG2 cells
Positive IP detected inmouse liver tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25953-1-AP targets C20orf3/APMAP in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen C20orf3/APMAP fusion protein Ag23029
Full Name chromosome 20 open reading frame 3
Calculated Molecular Weight 416 aa, 46 kDa
Observed Molecular Weight 42-46 kDa
GenBank Accession NumberBC003501
Gene Symbol APMAP
Gene ID (NCBI) 57136
RRIDAB_2880308
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for C20orf3/APMAP antibody 25953-1-APDownload protocol
IHC protocol for C20orf3/APMAP antibody 25953-1-APDownload protocol
IP protocol for C20orf3/APMAP antibody 25953-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF

Cancer Res

Cholesterol induces epithelial-to-mesenchymal transition of prostate cancer cells by suppressing degradation of EGFR through APMAP.

Authors - Siyuan Jiang
  • KD Validated
View Article
mouseWB

J Nutr Biochem

Thioredoxin domain containing 5 is involved in the hepatic storage of squalene into lipid droplets in a sex-specific way

Authors - Javier Sánchez-Marco
View Article