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ANKZF1 Polyclonal antibody

ANKZF1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20447-1-AP

Synonyms

ANKZF1, Zinc finger protein 744, ZNF744

Validation Data Gallery

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  • Western Blot (WB) analysis of BxPC-3 cells using ANKZF1 Polyclonal antibody (20447-1-AP)

    WB analysis of BxPC-3 using 20447-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 20447-1-AP (ANKZF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using ANKZF1 Polyclonal antibody (20447-1-AP)

    WB analysis of MCF-7 using 20447-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 20447-1-AP (ANKZF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ANKZF1 Polyclonal antibody (20447-1-AP)

    WB analysis of HeLa using 20447-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20447-1-AP (ANKZF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using ANKZF1 Polyclonal antibody (20447-1-AP)

    WB analysis of HepG2 using 20447-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20447-1-AP (ANKZF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using ANKZF1 Polyclonal antibody (20447-1-AP)

    WB analysis of HeLa using 20447-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20447-1-AP (ANKZF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using ANKZF1 Polyclonal antibody (20447-1-AP)

    WB analysis of COLO 320 using 20447-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 20447-1-AP (ANKZF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using ANKZF1 Polyclonal antibody (20447-1-AP)

    WB analysis of MCF-7 using 20447-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 20447-1-AP (ANKZF1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using ANKZF1 Polyclonal antibody (20447-1-AP)

    IHC staining of human liver using 20447-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20447-1-AP (ANKZF1 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using ANKZF1 Polyclonal antibody (20447-1-AP)

    IHC staining of human liver using 20447-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20447-1-AP (ANKZF1 antibody) at dilution of 1:200 (under 10x lens).

Tested Applications

Positive WB detected inBxPC-3 cells, COLO 320 cells, HeLa cells, HepG2 cells, MCF-7 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 20447-1-AP is ANKZF1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ANKZF1 fusion protein Ag14285
Full Name ankyrin repeat and zinc finger domain containing 1
Calculated Molecular Weight 726 aa, 81 kDa
Observed Molecular Weight 81 kDa
GenBank Accession NumberBC000238
Gene Symbol ANKZF1
Gene ID (NCBI) 55139
RRIDAB_2878688
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for ANKZF1 antibody 20447-1-APDownload protocol
IHC protocol for ANKZF1 antibody 20447-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Commun

Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis

Authors - Ji Geng
View Article
humanWB,IF

Acta Neuropathol Commun

Inefficient quality control of ribosome stalling during APP synthesis generates CAT-tailed species that precipitate hallmarks of Alzheimer's disease

Authors - Suman Rimal
View Article
humanWB

bioRxiv

Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeats

Authors - Yi-Ju Tseng
View Article
humanWB

Nucleic Acids Res

Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeats

Authors - Yi-Ju Tseng
View Article
humanWB,IHC

Oncol Rep

Overexpression of ZNF169 promotes the growth and proliferation of colorectal cancer cells via the upregulation of ANKZF1

Authors - Jie Zhang
View Article