Left: HeLa cells transiently expressing pSpot Spot-Tag?-Actin (ev-31) were immunostained with Spot-Label? Alexa Fluor? 568 (red, ebAF568, 1:800) and DAPI (blue). Right: Control staining with Spot-Label? Alexa Fluor? 568 (red, ebAF568, 1:800) and DAPI (blue) of untransfected HeLa cells showing no unspecific background. Scale bar, 10 μm. Images were acquired with the Thermo Scientific CellInsight CX7, 20X objective.