UNC119B Polyclonal antibody

UNC119B Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26535-1-AP

Synonyms

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Validation Data Gallery

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  WB analysis using 26535-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26535-1-AP (UNC119B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, Y79 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26535-1-AP targets UNC119B in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: UNC119B fusion protein Ag24916
• Full Name: unc-119 homolog B (C. elegans)
• Observed Molecular Weight: 28-32 kDa
• GenBank Accession Number: BC004815
• Gene Symbol: UNC119B
• Gene ID (NCBI): 84747
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

UNC119b is a myristoyl-binding protein that acts as a cargo adapter: specifically binds the myristoyl moiety of a subset of N-terminally myristoylated proteins and is required for their localization. The myristoyl-binding activity of UNC119b is required to localize NPHP3 to the primary cilium (PMID: 22085962). Mouse Unc119b shares 90% sequence identity with human UNC119b protein.