RGS1 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

RGS1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12547-1-PBS

Synonyms

IER1, Early response protein 1R20, BL34, B-cell activation protein BL34, B cell activation protein BL34

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Validation Data Gallery

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  WB analysis using 12547-1-AP (same clone as 12547-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12547-1-AP (RGS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12547-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 12547-1-AP (same clone as 12547-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12547-1-AP (RGS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12547-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 12547-1-AP (same clone as 12547-1-PBS)

  WB result of RGS1 antibody (12547-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-RGS1 transfected HepG2 cells. This data was developed using the same antibody clone with 12547-1-PBS in a different storage buffer formulation.

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  IHC staining of human stomach cancer using 12547-1-AP (same clone as 12547-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12547-1-AP (RGS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 12547-1-PBS in a different storage buffer formulation.

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Product Information

12547-1-PBS targets RGS1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RGS1 fusion protein Ag3315
• Full Name: regulator of G-protein signaling 1
• Calculated Molecular Weight: 196 aa, 22 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC015510
• Gene Symbol: RGS1
• Gene ID (NCBI): 5996
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

RGS1 (Regulator of G-protein signaling 1) is also named as 1R20, BL34 and IER1. RGS1 encodes a member of the regulator of G-protein signaling family, which can act as a GTPase-activating protein (GAP), increasing the rate of conversion of the GTP to GDP, driving G-protein into its inactive GDP-bound form, hence attenuating or turning off G-protein-coupled receptor signaling (PMID: 8774882). RGS1 is highly expressed in immune cells including T cells, B cells, natural killer (NK) cells, dendritic cells and monocytes, suggesting a role for RGS1 in immune cell regulation (PMID: 34956194). RGS1 was highly expressed in tumor tissues and correlated with shorter overall survival, which also appeared in several previous studies, including multiple myeloma, melanoma, nonsmall cell lung cancer, gastric cancer , diffuse large B-cell lymphoma (PMID: 34956194). RGS1 protein, located at the cytoplasm and membrane, is enriched in tumor tissues compared with normal tissues. RGS1 was identified as a HIF-dependent hypoxia target that dampens cell migration and signal transduction (PMID: 24686421).