PDIA2 Polyclonal antibody

PDIA2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 31458-1-AP

Synonyms

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Validation Data Gallery

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  WB analysis using 31458-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31458-1-AP (PDIA2 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse pancreas tissue, rat pancreas tissue

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31458-1-AP targets PDIA2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDIA2 fusion protein Ag35301
• Full Name: protein disulfide isomerase family A, member 2
• Calculated Molecular Weight: 58 kDa
• Observed Molecular Weight: 60-65 kDa
• GenBank Accession Number: NM_006849.2
• Gene Symbol: PDIA2
• Gene ID (NCBI): 64714
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDIA2 (Protein disulfide-isomerase A2), a member of PDI family, is an ER protein chaperone that catalyzes the formation and breakage of disulfide bonds between cysteine residues and can inhibit aggregation of misfolded proteins by catalyzing rearrangement of -S-S- bonds in proteins. (PMID: 23486466)