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  • KD/KO Validated

JAK2 Polyclonal antibody

JAK2 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17670-1-AP

Synonyms

JAK 2, JAK2, Janus kinase 2, JTK10, Tyrosine protein kinase JAK2



Tested Applications

Positive WB detected inJurkat cells, NIH/3T3 cells
Positive IP detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 17670-1-AP is JAK2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, rat, zebrafish, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen JAK2 fusion protein Ag11264
Full Name Janus kinase 2 (a protein tyrosine kinase)
Calculated Molecular Weight 1132 aa, 130 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC039695
Gene Symbol JAK2
Gene ID (NCBI) 3717
RRIDAB_2811138
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Janus kinases (JAKs) are non-receptor tyrosine kinases that are activated by cytokine binding to cytokine receptors resulting in stimulation of the JAK-STAT signaling pathway. Aberrant activation of this signaling pathway is associated with numerous immune disorders and cancer. This antibody can detect full-length band of 130 kDa, and another bands 150 kDa after posttranslational modification.

Protocols

Product Specific Protocols
WB protocol for JAK2 antibody 17670-1-APDownload protocol
IHC protocol for JAK2 antibody 17670-1-APDownload protocol
IP protocol for JAK2 antibody 17670-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Neuroinflammation

Kynurenic acid blunts A1 astrocyte activation against neurodegeneration in HIV-associated neurocognitive disorders

Authors - Jingxian Lun
humanWB,IP

Cell Death Dis

4EBP1 senses extracellular glucose deprivation and initiates cell death signaling in lung cancer

Authors - Yanan Wang
mouseWB

Theranostics

Combination therapy with ropivacaine-loaded liposomes and nutrient deprivation for simultaneous cancer therapy and cancer pain relief.

Authors - Jiqian Zhang
humanWB

Arch Med Res

Extracellular Vesicles from Carcinoma-associated Fibroblasts Promote EMT of Salivary Adenoid Cystic Carcinoma Via IL-6

Authors - Hyat Ahmed Ibrahim Al-Raimi
mouseWB

Oxid Med Cell Longev

Mild Hypothermia Attenuates Hepatic Ischemia-Reperfusion Injury through Regulating the JAK2/STAT3-CPT1a-Dependent Fatty Acid β-Oxidation.

Authors - Wei Wang
ratIF

Brain Behav Immun

Interleukin-6: Its role and mechanisms in rescuing depression-like behaviors in rat models of depression.

Authors - Peng Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Carly (Verified Customer) (11-17-2020)

Tested using EDTA plasma on an antibody microarray

  • Applications: Other