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IL-15 Monoclonal Matched Antibody Pair, PBS Only

Host / Isotype

Capture: Mouse / IgG2a

Detection: Mouse / IgG2a

Reactivity

Capture: Human

Detection: Human

Applications

Sandwich ELISA

Range

Sandwich ELISA: 1.953-62.5 ng/mL

Cat No : MP50095-1

Print datasheet

Synonyms

IL 15, IL15, interleukin 15

Validation Data Gallery

  • Sandwich ELISA standard curve of MP50095-1, IL-15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68742-1-PBS. Detection antibody: HRP-conjugated 68742-2-PBS. Standard:HZ-1323. Range: 1.953-62.5 ng/mL.

    Sandwich ELISA standard curve of MP50095-1

    Sandwich ELISA standard curve of MP50095-1, IL-15 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68742-1-PBS. Detection antibody: HRP-conjugated 68742-2-PBS. Standard:HZ-1323. Range: 1.953-62.5 ng/mL.

Tested Applications

Sandwich ELISA

Recommended dilution

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

MP50095-1 targets IL-15 in immunoassays as a matched antibody pair. Validated in Sandwich ELISA.

Range: 1.953-62.5 ng/mL (Sandwich ELISA);

Capture antibody: IL15 Monoclonal antibody, PBS Only (Capture) 68742-1-PBS (1H6D7). 100 μg. Concentration 1 mgl/ml.

Detection antibody: IL15 Monoclonal antibody, PBS Only (Detector) 68742-2-PBS (8H2G10). 100 μg. Concentration 1 mgl/ml.

Matched pair protein standard:

Unconjugated mouse monoclonal antibody pair in PBS only storage buffer at a concentration of 1 mg/mL, ready for conjugation.

Matched antibody pairs are designed for use in a variety of assays and platforms that require matched antibody pairs. These include:
Sandwich ELISA
Multiplex bead-based arrays
FRET/TR-FRET/HTRF
Simoa® immunoassays (registered trademark of Quanterix, Inc)
AlphaLISA® (registered trademark of PerkinElmer, Inc)
Meso Scale Discovery® (registered trademark of Meso Scale Diagnostics, LLC)
ELLA™ Automated Immunoassay (registered trademark of Bio-Techne Corporation)

Please note we have not tested this product in the above platforms, they are listed for suggestion only as applications for matched antibody pairs. Antibody use should be optimized for each application and assay.

Catalog Number 68742-1-PBS
Tested Reactivity Human
Immunogen IL15 fusion protein Ag3650
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Affinity
KD=9.26 x 10-12M
KOff=2.61 x 10-6M
KOn=2.82 x 105M
Full Name interleukin 15
Synonyms IL 15, IL15, interleukin 15
GenBank accession numberBC018149
Gene symbol IL15
UniprotP40933
Gene ID (NCBI) 3600
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.
Catalog Number 68742-2-PBS
Tested Reactivity Human
Immunogen IL15 fusion protein Ag3650
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Affinity
KD=1.46 x 10-11M
KOff=2.93 x 10-6M
KOn=2.01 x 105M
Full Name interleukin 15
Synonyms IL 15, IL15, interleukin 15
GenBank accession numberBC018149
Gene symbol IL15
UniprotP40933
Gene ID (NCBI) 3600
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS only
Storage Conditions Store at -80°C.