IKZF3 Polyclonal antibody, PBS Only

IKZF3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 19055-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of HepG2 using 19055-1-AP (same clone as 19055-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 19055-1-AP (IKZF3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19055-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 19055-1-AP (same clone as 19055-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19055-1-AP (IKZF3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19055-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 19055-1-AP (same clone as 19055-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19055-1-AP (IKZF3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19055-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 19055-1-AP (same clone as 19055-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 19055-1-AP (IKZF3 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19055-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 19055-1-AP (same clone as 19055-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 19055-1-AP (IKZF3 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19055-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HepG2 using 19055-1-AP (same clone as 19055-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using IKZF3 antibody 19055-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 19055-1-PBS in a different storage buffer formulation.

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Product Information

19055-1-PBS targets IKZF3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IKZF3 fusion protein Ag5537
• Full Name: IKAROS family zinc finger 3 (Aiolos)
• Calculated Molecular Weight: 509 aa, 58 kDa
• Observed Molecular Weight: 58-70 kDa
• GenBank Accession Number: BC032707
• Gene Symbol: IKZF3
• Gene ID (NCBI): 22806
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

IKZF3, also named as Zinc finger protein Aiolos or ZNFN1A3, is a 509 amino acid protein, which contains 6 C2H2-type zinc fingers and belongs to the Ikaros C2H2-type zinc-finger protein family. IKZF3 localizes in the nucleus and cytoplasm. IKZF3 can form homodimer, and heterodimer with other IKAROS family members. IKZF3 is expressed most strongly in peripheral blood leukocytes, the spleen, and the thymus. IKZF3 as a transcription factor plays an important role in the regulation of lymphocyte differentiation. The calculated molecular weight of IKZF3 is 58 kDa, but the modified IKZF3 protein is about 65-70 kDa.