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ICOS Polyclonal antibody, PBS Only

ICOS Polyclonal Antibody for WB, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF-P, Indirect ELISA

Conjugate

Unconjugated

Cat no : 13338-1-PBS

Synonyms

Inducible T cell costimulator, inducible T cell co stimulator, CVID1, CD278, AILIM

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using ICOS Polyclonal antibody (13338-1-AP)

    WB analysis of Jurkat using 13338-1-AP (same clone as 13338-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13338-1-AP (ICOS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13338-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HL-60 cells using ICOS Polyclonal antibody (13338-1-AP)

    WB analysis of HL-60 using 13338-1-AP (same clone as 13338-1-PBS)

    HL-60 cells were subjected to SDS PAGE followed by western blot with 13338-1-AP (ICOS Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13338-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using ICOS Polyclonal antibody (13338-1-AP)

    IF Staining of human tonsillitis using 13338-1-AP (same clone as 13338-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using ICOS antibody (13338-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13338-1-PBS in a different storage buffer formulation.

Product Information

13338-1-PBS targets ICOS in WB, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ICOS fusion protein Ag4155
Full Name inducible T-cell co-stimulator
Calculated Molecular Weight 199 aa, 23 kDa
Observed Molecular Weight 28 kDa, 45-50 kDa
GenBank Accession NumberBC028210
Gene Symbol ICOS
Gene ID (NCBI) 29851
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Inducible T cell co-stimulator (ICOS) is related to the CD28 superfamily and is highly expressed on activated T cells as well as regulatory T cells and is crucial for the survival and function of T cells, Th2 cell differentiation, and lung inflammatory responses. Binding ICOS to ICOS-ligand (ICOS-L) activates a cascade of intracellular signaling molecules that prevent apoptosis and lead to the production of cytokines such as IL-4 and IL-13. Catalog#13338-1-AP recognizes two bands of 25-30 kDa and 45-50 kDa, and the additional 45-50 kDa band may be due to  surface disulfide-linked homodimeric glycoprotein.